Products
Pathological Reagents | Consumables
Fixatives
Common Samples
Paraformaldehyde Fixative (Neutral)
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หมวดหมู่ : Biochemicals , 1. Chemical and Reagents , Buffers , Servicebio ,
แบรนด์ : Servicebio
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Product Information
Product Name | Cat.No. | Spec. | Packaging |
Paraformaldehyde Fixative (Neutral) | G1101-3ML | 3 mL | Single pre-filled, ready to use. Blister box, 50 pcs/box, 8 boxes/ctn. |
G1101-15ML | 15 mL | Single pre-filled, ready to use. Blister box, 50 pcs/box, 8 boxes/ctn. | |
G1101-25ML | 25 mL | Single pre-filled, ready to use. Blister box, 50 pcs/box, 12 boxes/ctn. | |
G1101-500ML | 500 mL | Bottle, 25 bottles/ctn. | |
G1101-25L | 25 L | Barrel-loading |
Description
In histopathology, tissues must be fixed in a timely and effective manner before subsequent embedding and sectioning can be completed. The role of tissue fixation is to maintain the inherent form and structure of tissues and cells, prevent bacterial corrosion and autolysis of tissue cells, preserve the inherent substances in cells, coagulate intracellular proteins, minimize or terminate the reaction of endogenous and exogenous enzymes, so that cells or tissues basically remain the same as the substances in life, and also make tissues harden, which is convenient for tissue cutting. For some infectious specimens, it can prevent the spread of disease; Keep rough specimens. Finally, fixation enhances the effect of staining. Formaldehyde is the most commonly used fixative, with strong penetration, uniform fixation, small tissue shrinkage and appropriate hardness, which is suitable for the fixation of most tissue samples.
This product is a universal tissue fixative solution (neutral) with an active ingredient of 4% paraformaldehyde with 0.01 M phosphate buffer as the solvent, pH 7.0-7.5@25°C. This product is suitable for the fixation of most animal tissues and also for the fixation of flow assay cells.
This product provides a variety of specifications of packaging to meet different needs of use. Among them, the single pre-filled type is already dispensed, ready to use, you can directly put the tissue sample into the tube, convenient and quick. Transparent tube body for easy observation of the sample. Leak-proof design ensures safe use and transportation. Pre-labeled for easy and timely recording of sample information. Barrels are suitable for scenarios with larger dosage.
Storage and Shipping Conditions
Transportation at room temperature; keep in cool and dry place, avoid direct sunlight, valid for 24 months.
Assay Protocol / Procedures
Freshly taken tissue specimens quickly submerged in the product, the volume ratio of fixative to tissue block is not less than 10:1, the tissue block should not be too thick, 5 mm is the best, the fixation time is generally 1-4 h/mm, or fixation for 24 h can be carried out for the subsequent operation, large specimens can be appropriately extended fixation time.
In vitro cultured cell crawls were fixed for 15 min or less.
Note
1. Use in a well-ventilated environment and protect against inhalation.
2. To ensure adequate penetration of the fixative into the tissue, do not take tissue thicker than 5 mm and ensure that the volume of fixative is sufficient. If the volume is not large enough, change the fixative 2-3 times during the fixation period.
3. Please tighten the cap promptly after use to prevent the active ingredients from evaporating.
4. For your safety and health, please wear a lab coat and disposable gloves during operation.
For Research Use Only!
