Citric Acid Repair Buffer (Dry Powder)
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หมวดหมู่ : Biochemicals , 1. Chemical and Reagents , Buffers , Servicebio ,
แบรนด์ : Servicebio
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Product Information
Product Name | Cat. No. | Spec.. |
Citric acid Antigen Retrieval Solution (dry powder, pH 6.0) | G1201-1L | 1 L |
G1201-5L | 5 L |
Product Description/Introduction
After cells or tissues are fixed with paraformaldehyde, formalin or other aldehyde reagents, the formation of aldehyde bond and carboxymethyl bond of antigen determinant, as well as the cross-linking of protein quality control, cause the change of protein spatial structure, so that the antigen determinant is closed, and the binding point between antigen and antibody is reduced. Finally, the positive detection rate and staining intensity of antigen during immune labeling are relatively weak, showing false negative or low positive rate. The protein cross-linking caused by aldehydes is reversible. Under certain conditions, such as high temperature or protease hydrolysis, the original conformation of the protein can be restored. This process is antigen retrieval. Antigen repair is a key step in immunohistochemistry. There are many methods for antigen repair, and different methods can be selected according to the characteristics of the tissue type. Some are suitable for thermal repair, while others are suitable for enzymatic repair. The pH value of antigen repair solution also has many different choices due to the characteristics of antigen. The company provides a variety of antigen retrieval solutions with different pH values, which can meet the needs of a variety of antigen repair.
This product is a dry powder citric acid antigen retrieval solution. Citric acid buffer with pH 6.0 and concentration of 10 mM can be obtained after being dissolved in pure water. It can be used for antigen retrieval.
Storage and Shipping Conditions
Room temperature storage and transportation; Valid for 24 months.
Assay Protocol / Procedures
For 1 L specification of citric acid antigen retrieval liquid powder, dissolved in 1000 mL water clarification, then it can be used.
For 5 L specification of citric acid antigen retrieval liquid powder, dissolved in 5000 mL water clarification, then it can be used.
Note
1. Use as soon as possible after dissolution. Long-term storage is not recommended.
2. Please wear a lab coat and disposable gloves during operation.
For Research Use Only!
