Agarose Amine Magnetic Beads (SweMagrose NH2)

Product Information

Product Description/Introduction

Magnetic separation technology can easily separate or enrich antibodies, antigens, lectins, proteases, nucleic acids and cells while maintaining their biological activity. SweMagrose NH2 perfectly fused superparamagnetic materials and polymer materials together by polymer polymerization technology to form a suspension of superparamagnetic iron oxide microspheres, which was coated with carboxyl groups. Compared with traditional magnetic beads, SweMagrose NH2 has faster magnetic response, good dispersion, very low non-specific adsorption and more abundant binding sites. SweMagrose NH2 can efficiently bind to a variety of biological ligands (oligonucleotides, peptides, proteins, drug molecules, etc.) under the action of special reagents (such as glutaraldehyde). It is a good basic material for subsequent treatment, such as coating, adsorption, chemical modification and so on.

Abundant binding sites: Enhances specific binding to ligand

High Magnetic Response: Faster magnetic response saves experiment time.

Superparamagnetic: good dispersion even after the disappearance of the external magnetic field.

Excellent dispersibility and resuspension: good dispersibility and resuspension ensure the stability of the experiment and facilitate the operation.

Excellent physicochemical stability: better guarantee of experimental reproducibility.

Product Components

Storage and Shipping Conditions

Ship at room temperature; Store at 2-8，valid for 24 months.

Product Components

Assay Protocol/Procedures (Take coupling protein G as an example)

1. Self-provided reagent preparation

PBS solution(pH 7.4): 2.7 mM KCl，2.0 mM KH2PO4，137 mM NaCl，10 mM Na2HPO4，pH 7.3-7.5@25(recommended G4202-100ML)；

0.1 M carbonate buffer (pH 9.6): 0.1 M sodium carbonate and 0.1 M sodium bicarbonate solutions were prepared separately, and the pH of the 0.1 M ammonium carbonate solution was adjusted to PH 9.6 with 0.1 M sodium bicarbonate solution.

Glutaraldehyde solution: 45 μL of 25% aqueous glutaraldehyde was added to 55 mL of 0.1 M carbonate buffer (pH 9.6) and mixed.

2. Magnetic bead pretreatment: After mixing the agarose-amino magnetic beads (SweMagrose NH2), 100 μL of the beads were taken into a 1.5 mL EP tube and placed on a magnetic separation rack for 30 s. The beads were washed three times with 500 μL of PBS solution (50 mM PBS, pH 7.4), and the supernatant was aspirated by magnetic separation.

3. Activation of glutaraldehyde: Add 100 μL of freshly prepared glutaraldehyde solution (45 μL of 25% glutaraldehyde + 55 mL of pH=9.6 0.1 M carbonate buffer) to the EP tube, vortex and mix to fully suspend the beads, wrap in tin foil and activate for 1 h at 25°C (keep the reaction out of the light to avoid polymerisation of the glutaraldehyde itself), keep the beads suspended during this period. (vertical mixer can be used to invert the mixing);

4. Post-activation wash: After activation of the beads, the supernatant was removed by magnetic suction and washed 3 times with PBS buffer.

5. Magnetic bead coupling: Add 50~200 μg of protein to the EP tube containing magnetic beads (concentration and dosage should be optimised according to the specific conditions of the experiment, here we recommend using 0.1 M carbonate buffer (pH 9.6) and adding 0.05% Tween-20 to improve dispersibility), mix gently, wrap in tin foil and then react at 25°C for 3 h (avoiding light), or react at 25°C for 1 h and then keep the beads suspended at 4°C overnight. 1 h and then coupled at 4°C overnight, keep the beads in suspension during coupling. 

6. Post-coupling closure: Place the EP tube on a magnetic separation rack to remove the supernatant, add 500 μL of closure solution (5% (w/v) BSA dissolved in PBS can be used, e.g., 5 g of BSA in 100 mL of PBS), and react for 1h at 25°C, keeping the beads suspended during the reaction.

7. Preservation: magnetically aspirate the supernatant, wash three times with 200 mL of PBS or preservation solution, control the concentration of magnetic beads according to the actual need, and store it at 4°C. Add 0.02% (w/v) sodium azide if necessary.

Note

1. Operations such as freezing, drying and centrifugation cause agglomeration of the beads, which makes them difficult to resuspend and disperse and affects the chemical activity of the functional groups on the surface of the beads.

2. The product is stored in 20% ethanol. Wash the beads 2-3 times with pure water or buffer to remove ethanol from the preservation solution before use.

3. Be sure to shake or sonicate the beads well before using the product so that they are evenly suspended.

4. Use with a matching magnetic holder.

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