Agarose Carboxyl Magnetic Beads (SweMagrose COOH)

Product Information

Product Description/Introduction

Magnetic separation technology can easily separate or enrich antibodies, antigens, lectins, proteases, nucleic acids and cells while maintaining their biological activity. Magnetic agarose carboxylated beads (SweMagrose COOH) use polymer polymerisation technology to blend superparamagnetic materials and polymers together to make a suspension of superparamagnetic iron oxide microspheres with carboxylate groups on the surface; SweMagrose COOH has faster magnetic responsiveness, good dispersion, extremely low non-specific adsorption, and richer binding sites than traditional magnetic beads. Compared with traditional magnetic beads, SweMagrose COOH has faster magnetic responsiveness, good dispersion, extremely low non-specific adsorption and richer binding sites; SweMagrose COOH can efficiently bind with a variety of biological ligands (oligonucleotides, peptides, proteins, drug molecules, etc.) at high loads with special reagents (e.g., EDC), which is the best way to carry out the subsequent treatments such as encapsulation and adsorption, It is a good base material for subsequent processing such as encapsulation, adsorption, chemical modification, etc.

Abundant binding sites: Enhances specific binding to ligand

High Magnetic Response: Faster magnetic response saves experiment time.

Superparamagnetic: good dispersion even after the disappearance of the external magnetic field.

Excellent dispersibility and resuspension: good dispersibility and resuspension ensure the stability of the experiment and facilitate the operation.

Excellent physicochemical stability: better guarantee of experimental reproducibility.

Product Information

Storage and Shipping Conditions

Ship at room temperature; Store at 2-8，valid for 24 months.

Product Components

Assay Protocol/Procedures(Take coupling protein G as an example)

A. Self-provided reagent preparation

MEST Solution: MES (2-morpholine ethanesulfonic acid) 100 mM, 0.05% Tween 20, adjust pH to 5.0 with sodium hydroxide.

EDC (carbodiimide) solution: EDC 10 mg/mL dissolved in MEST solution.

NHS (N-hydroxysuccinimide) solution: NHS 10 mg/mL dissolved in MEST solution.

B. Carboxyl group activation on magnetic bead surface

a) After mixing the agarose carboxylated magnetic beads (SweMagrose COOH), take 100 µL into a 1.5 mL EP tube and place it on a magnetic separation rack for 30 s. Remove the supernatant, wash it twice with 200 µL of MEST solution by magnetic separation, and then aspirate the supernatant.

b) Rapidly add 100 µL of freshly prepared EDC solution and 100 µL of NHS solution to the centrifuge tube containing the beads, vortex to fully suspend the beads, activate for 30 min at 25°C, keep the beads in suspension during this period (vertical mixer can be used for inverted mixing); after the above steps the carboxyl groups on the surface of the beads are activated and ready for covalent coupling with biological ligands with primary amino groups (the activated state is not suitable for prolonged storage, immediate coupling is recommended).

C. Covalent coupling of magnetic beads to biological ligands

a) The supernatant was removed by magnetic suction, add 50~200 µg of biological ligand (dosage, concentration and type of buffer need to be optimised according to the specific experiments, and the following ligand buffers can be used: 100 mM 2-morpholine ethanesulfonic acid buffer, pH 4.8; 200 mM sodium bicarbonate buffer, pH 8.3; 50 mM phosphate buffer, pH 8.5; 100 mM sodium chloride solution, pH 7.4, etc.; 0.05% Tween 20 may be added to the buffer to improve the dispersion of the beads and to avoid the presence of reagents containing primary amines other than biological ligands in the buffer system), mix gently.

b) Couple at 25°C for 2 h or 25°C for 1 h and leave overnight at 4°C, keeping the beads in suspension during coupling (vertical mixer can be used for inverted mixing)

c) The centrifuge tube is placed in a magnetic separation rack and the supernatant is removed by magnetic suction, the beads are resuspended (sonicated if required) in 200 µL of PBST solution (pH 7.2 with 1% BSA) and reacted at 25°C for 1 h to seal off unreacted activated carboxyl groups on the surface of the beads, which are kept in suspension during this time (inverted mixing can be carried out using a vertical mixer).

d) Centrifuge tubes were placed in a magnetic separation rack and the supernatant was removed by magnetic suction separation, washed three times and each time with 200 µL of PBS solution (pH 7.2) or preservation solution, and then re-suspended in preservation solution (the amount of preservation solution added can be determined as needed to adjust the concentration of coupled ligand beads) and stored at 4°C; if the immobilised biologic ligands are stable, 0.02% (w/v) sodium azide (NaN3) can be added to preservation solution as a bacteriostatic inhibitor.

Note

1. Operations such as freezing, drying and centrifugation cause agglomeration of the beads, which makes them difficult to resuspend and disperse and affects the chemical activity of the functional groups on the surface of the beads.

2. The product is stored in 20% ethanol. Wash the beads 2-3 times with pure water or buffer to remove ethanol from the preservation solution before use.

3. Be sure to shake or sonicate the beads well before using the product so that they are evenly suspended.

4. Use with a matching magnetic holder.

For research use only. Not for use in diagnostic or therapeutic procedures!

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