Recombinant Firefly Luciferase, 1 mL (for Industrial raw protein/enzyme)

Product Information

Product Introduction

This product, Recombinant Firefly Luciferase, is a luciferase derived from the North American firefly ( Photinus pyralis ) expressed by Escherichia coli , with a molecular weight of about 62 kDa. This enzyme requires ATP, molecular oxygen and the heterocyclic compound luciferin to produce light through a two-step reaction. Luciferase is widely used in molecular and cell biology, mainly for the detection of quantitative ATP using bioluminescent reagents or as an essential raw material for luciferase research and kit production using luciferin as a substrate.

Definition of enzyme activity: The amount of enzyme required to produce one relative light unit (RLU) within 10 s at 20-25°C is defined as one unit of enzyme activity.

Purity and concentration: Purity detected by SDS-PAGE 95%, 1 mg/mL.

Enzyme Storage Buffer: 20 mM Tris-HCl, 1 mM DTT, 50% glycerol, pH 8.0.

Storage and transportation

Transported in wet ice packs; stored at -20°C, valid for 12 months.

composition

Precautions

1. Enzyme products should be placed in an ice box or on an ice bath when in use, and should be divided into smaller packages for use. After use, they should be immediately stored at -20°C.

2. If the enzyme needs to be diluted, it is recommended to use enzyme storage buffer for dilution.

3. For your safety and health, please wear a lab coat and disposable gloves when operating.

The product is for scientific research purposes only and not for clinical diagnosis!

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