10×TE Buffer (pH 8.0, Sterile, DNase, RNase & Proteinase free)

10×TE Buffer (pH 8.0, Sterile, DNase, RNase & Proteinase free)
Its weak alkaline properties, provides better preservation for DNA. The presence of EDTA in TE buffer helps to reduce the risk of DNA degradation by potential residual DNase enzymes.

Product Information

Product Description/Introduction
TE Buffer, also known as Tris-EDTA buffer, is a concentrated solution used for various applications involving DNA or RNA samples. This product, 10× TE Buffer (pH 8.0, Sterile, DNase, RNase & Proteinase-free), is a sterile, concentrated Tris-EDTA buffer that is free from DNase, RNase, and proteinase contamination. It contains 100 mM Tris, 10 mM EDTA, with a pH of 8.0±0.1 at 25ºC.
To obtain a ready-to-use TE buffer with a final concentration of 10 mM Tris and 1 mM EDTA at pH 8.0 @ 25ºC, this product can be diluted 10-fold with sterile, enzyme-free ultrapure water. It can be used for sample dissolution, dilution, or treatment involving DNA or RNA samples.
This product is prepared using EDI ultrapure water and high-purity Tris, sterilized through a 0.22 μm filtration process, and has been tested to ensure the absence of nucleases and proteinase contamination.

Storage and Shipping Conditions
Room temperature transportation; store at room temperature. If not in use for an extended period, it can be stored at 2-8°C.

Assay Protocol / Procedures
1.During operation, strict attention must be paid to avoiding RNase contamination and microbial contamination. It is recommended to dispense and dilute the solution within a clean bench while wearing a mask and gloves.
2.The pH of the Tris solution is sensitive to temperature and should be equilibrated to room temperature before use.
This product is for research use only and is not intended for clinical diagnosis.