High Purity Plasmid DNA Miniprep Kit
คุณสมบัติสินค้า:
SKU : G3630-100T
Nucleic Acid Extraction >DNA Extraction >Column-based│Servicebio
หมวดหมู่ : 1. Chemical and Reagents , Servicebio ,
แบรนด์ : Servicebio
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High Purity Plasmid DNA Miniprep Kit
Modified SDS-Alkaline Lysis Method for Plasmid DNA Extraction
Product Information
| Product Name | Cat. No. | Spec. | Notes |
| High Purity Plasmid DNA Miniprep Kit | G3630-100T | 100 T | 1.High purity, extracted plasmid DNA can be directly used for transfection and other-high demand experiments. 2.Up to 25μg high purity plasmid DNA can be extracted from 1-5 mL of bacterial liquid. 3.Equipped with indicators to indicate whether resuspension, lysis,and neutralization are complete, ensuring the quality of plasmid extraction. |
Product Description/Introduction
This kit adopts the improved SDS-base lysis method, the optimized lysis solution can make the centrifugal adsorption column specifically bind DNA in solution under high salt state, combined with the advanced silica gel membrane adsorption technology, it can achieve the purpose of rapid purification of plasmid DNA. Suitable for extracting up to 25 μg of high-purity plasmid DNA from 1-5 mL of bacterial culture. This kit removes impurity proteins and other organic compounds from cells. The rate and quality of plasmid extraction is related to host bacteria species and culture conditions, cell lysis, plasmid copy number, plasmid stability, antibiotics and other factors. The solution contains indicators to indicate whether resuspension, lysis, and neutralization are complete by its color change, so as to ensure the quality of plasmid extraction and visualize the operation process. Plasmid DNA extracted using this kit can be applied to enzymatic digestion, PCR, sequencing, ligation, transformation, library screening, in vitro translation and so on.
Storage and Shipping Conditions
RNase A is shipped with wet ice and stored at -20°C; the rest of the reagents are shipped and stored at room temperature; valid for 12 months.
