BioFact™2X Pfu PCR Pre-Mix, Standard (w/o Band Helper™), with dye, total volume 30 ul reaction, 8 strip x 12ea

BioFact™ Pfu DNA Polymerase, a product of recombinant type DNA polymerase from Pyrococcus furiosus, is a suitable product for high-fidelity PCR amplification. The error rate of BioFact™ Pfu DNA Polymerase is 12 times lower than BioFact™ S-Taq DNA Polymerase due to its intrinsic 3’ to 5’ exonuclease activity. It produces blunt ended PCR products and it enables blunt cloning directly using BioFact™ All-in PCR Cloning Kit without A-tailing step. Providing optional 5X Band Helper™ helps amplification of difficult PCR caused by High GC content or complex structure of template. 

• Recombinant type DNA polymerase
  
• High fidelity
  
• Low error rate
  
• Produces blunt ended PCR

FIGURES (Q,C)

DETAILED INSTRUCTIONS

FEATURES

• Source : Pyrococcus furiosus
  
• 5‘ → 3’ exonuclease activity : No
  
• 3‘ → 5’ exonuclease activity : Yes
• Amplification size : < 3 kb
  
• A-tailing : No
  
• Error rate : 1-2 bp error/ 106 bp

APPLICATION

• Routine PCR
  
• PCR screening
• TA cloning
  
• Genotyping (SNP, SSR, AFLP, T-RFLP and etc.)

STORAGE TEMPARATURE

• -20℃ (Stable for 7 days at 25℃)

EXPIRATION DATE

• 2 years and 3 months
  (in case of storage at -20℃)

http://bio-ft.com/en/pro1_1_09