5X All-In-One RT MasterMix

Cat. No. : G592Print
Name : All-In-One 5X RT MasterMix
Unit : 100 rxn
Category : Reverse Transcriptase & RT-PCR
Description : Enjoy accurate gDNA-free cDNA synthesis!
All-In-One 5X RT MasterMix is a convenient and ready-to-use formulation for first-strand cDNA synthesis, including genomic DNA (gDNA) removal. Genomic DNA contamination is a common problem for accurate RNA detection and this MasterMix solves that problem without affecting reverse transcription and first-strand cDNA synthesis. cDNA synthesis will be simple, reliable, and reproducible. The optimized 5X RT MasterMix contains abm’s proprietary OneScript® Hot Reverse Transcriptase, RNaseOFF Ribonuclease Inhibitor, temperature-sensitive DNase, dNTPs, and a finely-balanced ratio of Oligo (dT)s and Random Primers. The high-quality cDNA synthesized from this kit can be used for a wide range of downstream applications.

Product Features:

• Easy one-step setup reduces pipetting and sample handling
• High cDNA yields from difficult samples with OneScript® Hot Reverse Transcriptase (Cat. No. G593)
• Inhibits ribonuclease contaminants with RNaseOFF Ribonuclease Inhibitor (Cat. No. G591)
  
• Removes contaminating gDNA with temperature-sensitive DNase
  
• Also includes dNTPs, Oligo (dT)s, and random primers
  
• Earn 2X the rewards points

Application

• Generation of templates for use in RT-PCR and qRT-PCR
  
• cDNA synthesis from ssRNA
  
• cDNA library construction
  
• Generation of probes for hybridization
  
• DNA primer extension
  

Concentration : 5X
Storage Condition :Store at -20°C.
Material Citation : If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G592

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