Recombinant Mouse SIRP alpha Protein (His-tag)

Gene Name Synonym

CD172a, BIT; MFR, MYD1, MYD-1, P84, PTPNS1, SHPS1, SHPS-1, SHPS1CD172A, SIRPA, Sirp-alpha-1, SIRPalpha2, Sirp-alpha-2, Sirp-alpha-3

Species

Mouse

Accession

P97797

Source

HEK293 cells

SPECIFICATIONS

Amino acid sequence

Lys32-Asn373 is expressed with a 12×His tag at the C terminus

Molecular Weight

37.9 kDa（The protein migrates as 40-55 kDa under reducing condition (SDS-PAGE) due to glycosylation）

Purity

>95.0% as determined by SDS-PAGE

Endotoxin

<5 EU mg^-1

Formulation

Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "dont eat me" signal.Cancer cells highly expressed CD47 that activate SIRP α and inhibit macrophage-mediated destruction.

Data

SDS-PAGE

Bioactivity

Testing in progress

Recombinant Mouse SIRP alpha Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 45-90 kDa.