Recombinant Mouse TIM3 Protein (His-tag)

Gene Name Synonym

HAVCR2, TIM3, TIMD3, FLJ14428, KIM3, T-cell immunoglobulin mucin family member 3, TIM-3, CD366

Species

Mouse

Accession

Q8VIM0

Source

HEK293 cells

SPECIFICATIONS

Amino acid sequence

Arg20-Ala193 is expressed with a 10×His tag at the C terminus

Molecular Weight

19.3 kDa（The protein migrates as 20-40 kDa under reducing condition (SDS-PAGE) due to glycosylation）

Purity

>95.0% as determined by SDS-PAGE

Endotoxin

<5 EU mg^-1

Formulation

Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

Hepatitis A virus cellular receptor 2 is also known as HAVCR2, FLJ14428, KIM3, TIM3, TIMD3, is a member of the TIM family of immune regulating molecules with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. HAVCR2 is a Th1-specific cell surface protein that regulates macrophage activation and enhances the severity of experimental autoimmune encephalomyelitis in mice. HAVCR2 regulates macrophage activation. Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. May be also involved in T-cell homing. Dysregulation of the HAVCR2-galectin-9 pathway could underlie chronic autoimmune disease states in human, such as multiple sclerosis.

Data

SDS-PAGE

Bioactivity

Testing in progress

Recombinant Mouse TIM3 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 30-50 kDa.