Recombinant Human CD333 Protein (His-tag)

Gene Name Synonym

FGF R3 (IIIc), FGF R3C, CD333

Species

Human

Accession

P22607

Source

HEK293 cells

SPECIFICATIONS

Amino acid sequence

Glu23-Gly375 is expressed with a 8×His tag at the C terminus

Molecular Weight

42.1 kDa

Purity

>95.0% as determined by SDS-PAGE

Endotoxin

<5 EU mg^-1

Formulation

Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

Fibroblast growth factor receptors (FGFR) are transmembrane kinase proteins with growing importance in cancer biology given the frequency of molecular alterations and vast interface with multiple other signaling pathways. The FGFR3 gene is at the human chromosomal locus 4p16.3 also encodes two isoforms: FGF R3 (IIIb) FGF R3 (IIIc). Upregulated expression of FGFR3 protein is also found in a significant number of tumors. Additionally, FGFR3 has been found to trigger resistance to tamoxifen via the activation of the PLCγ signaling cascade.

Data

SDS-PAGE

Bioactivity

Testing in progress.

Recombinant Human CD333 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 60-100 kDa.