Recombinant Human CD74 Protein (His-tag)

Gene Name Synonym

CD74, DHLAG, HLADG, II, Ia-GAMMA, p33, CLIP, INVG34

Species

Human

Accession

P04233

Source

HEK293 cells

SPECIFICATIONS

Amino acid sequence

Gln73-Met296 is expressed with a 10×His tag at the N terminus

Molecular Weight

25.3 kDa（The protein migrates as 20~40 kDa under reducing condition (SDS-PAGE) due to glycosylation）

Purity

>95.0% as determined by SDS-PAGE

Endotoxin

<5 EU mg^-1

Formulation

Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

CD74 (MHC class II invariant chain, Ii) is a non-polymorphic type II transmembrane glycoprotein. CD74 has a diversity of biological functions in physiological and pathological situations.  CD74 is expressed in a number of cell types independently of MHC class II. CD74 is a cell membrane high-affinity receptor for macrophage migration inhibitory factor (MIF), D-dopachrome tautomerase (D-DT/MIF-2) and bacterial proteins. CD74 regulates T-cell and B-cell developments, dendritic cell (DC) motility, macrophage inflammation, and thymic selection. CD74 plays important roles in many inflammatory diseases, such as liver fibrosis, type I diabetes, systemic lupus erythematosus, and Alzheimer disease. CD74 may be a therapeutic target to treat the relevant immune disorders in the near future.

Data

SDS-PAGE

Bioactivity

Active Recombinant Human CD74 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 35-40 kDa.

Immobilized Human CD74, His Tag at 5 μg/mL (100 μL/well) can bind Anti-CD74 with a linear range of 0.02-0.15 μg/mL.