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Cell Biology Reagents | Consumables
Auxiliary reagents
Cell Dissociation
Recombinant Trypsin (Powder)
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Categories : 2. Cell & Molecular Biology , Cell Culture Medium / Sera , Servicebio ,
Brand : Servicebio
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Product Information
Product Name | Cat. No. | Spec. |
Recombinant Trypsin（Powder） | G3440-10MG | 10 mg |
GG3440-100MG | 100 mg |
Product Description
Trypsin is a serine protease that specifically cleaves lysine and arginine C-terminal peptide bonds in proteins. When the amino acid following lysine and arginine is proline, the cleavage activity of trypsin decreases.
Recombinant Trypsin ( Powder ) of this product was expressed and purified in Pichia pastoris using trypsin gene from pig. It did not contain endogenous nuclease and other protease activities. The molecular weight was about 25 kDa, and its purity was single main band detected by SDS-PAGE. Its effective temperature is 20-60°C, the optimum temperature is 35°C ; the effective pH range is 7-10, and the optimum pH is 8.5. It can replace the natural extraction of trypsin used in various biotechnology processes, such as : recombinant insulin production, cell culture, cell fermentation, protein enzymolysis, recombinant trypsin in addition to enzyme activity than the natural extraction of trypsin, the purity is also higher, and no other protease activity.
Enzyme activity unit and enzyme activity: The reaction conditions are 25°C, pH 8.0, and the reaction system is 3.2 mL. An enzyme activity unit U was defined as an increase of 0.003 in the absorbance of the reaction system at 253 nm by enzymatic hydrolysis of BAEE per minute. Enzyme activity 3800 U/mg (BAEE method).
Inhibitors: Its activity is inhibited by serine protease inhibitors (such as PMSF), and heavy metal ions can also inhibit its enzyme activity.
Storage and Shipping Conditions
Ship with wet ice; Store at -20°C for 24 months.
Product Component
Component | G3440-10MG | G3440-100MG |
Recombinant Trypsin（Powder） | 10 mg | 100 mg |
specification | 1 | 1 |
Assay Protocol/Procedures
Redissolution of Recombinant Trypsin lyophilized powder: Weigh appropriate amount of recombinant trypsin lyophilized powder and use it after completely dissolving in enzym-free water or other buffer solution. Recombinant Trypsin. After resolution, the product will slowly degrade after being placed at room temperature for several hours. It is recommended to store at -20.
Note
1. The redissolved trypsin will slowly degrade at room temperature. It is recommended to subpackage and store at -20°C.
2. For your safety and health, please wear a lab coat and disposable gloves.
For Research Use Only!
