Mycoplasma Elimination Reagent (SweMyco-1+2) (100×), 5 mL

Product Information

Product Description/Introduction

This product is used for cellular mycoplasma removal and is a combination reagent of quinolones, tetracyclines and macrolides, which provides effective removal of cellular mycoplasmas by inhibiting mycoplasma DNA replication and protein synthesis. Through optimization and modulation, this product not only has low cytotoxicity and fast mycoplasma removal rate, but also can completely remove mycoplasma contained in cultured cells in about 7 days, and after replacing the normal medium, the cells are in good condition, and there is no repositive phenomenon in continuous multi-generation culture.

Storage and Shipping Conditions

Ship with wet ice; store at -20, avoid freeze-thaw cycles, valid for 12 months.

Product Contents

Assay Protocol / Procedures

1. Preparation of medium A: Add 1 mL SweMyco Elimination Reagent-1 (100×) to 100 mL cell media (containing serum, without double antibiotic), and mix well.

2. Preparation of medium B: add 1 mL SweMyco Elimination Reagent 2 (100×) to 100 mL cell media (containing serum, without double antibiotic), and mix well.

3. When mycoplasma-infected cells were passaged or changed, cultivate cells in culture medium A for 4 days. And then the cells are conventionally cultured for 3 days with medium B.

4. After one round of culture (7 days), planting some cells into 6-well plates (or other standard culture vessels) and replaced with complete medium without SweMyco Elimination Reagent. The remaining cells are continued to be cultured with medium A and B alternately.

5. Cells in 6-well plates are cultured with SweMyco Elimination Reagent-free medium for 2-3 days, and mycoplasma detection (recommended G1900, G1901, G1902) is performed when the cell confluence reaches more than 90%.

6. According to the test result, perform the following operations:

7. A) Positive result: continue to repeat steps 3-5 until the test result is negative.

8. B) Negative results: the cell group containing Mycoplasma Elimination Reagent medium is replaced with normal complete medium, and Mycoplasma detection is performed after a period of culture; A negative retest indicates that mycoplasma elimination is complete.

Note

1. Avoid repeated freezing and thawing. If you need to use multiple times, please pay attention to appropriate packaging. In addition, if there is crystallization or precipitation after thawing, vortex or reverse mixing can promote crystallization or precipitation dissolution, without affecting the use effect.

2. This product has been tested in a variety of mycoplasma infected cell lines, but different cell lines have different sensitivity to reagents. If the cytotoxicity is too great or the removal effect is not good, the concentration of the product can be adjusted appropriately, or extend the removal time.

3. It is recommended to add mycoplasma prevention reagent (recommend G4009) in cell cultures to prevent mycoplasma contamination.

4. For cells that have been identified as mycoplasma contamination, a separate ultra-clean bench should be used to avoid cross-contamination.

5. For your safty and health, please wear safety glasses, gloves, or protective clothing.

Attached Table: Features of Mycoplasma Removal Series

For Research Use Only!

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