DMEM, high glucose, Powder, GlutaPlus, sodium pyruvate, no HEPES

Attribute:

SKU :

Option name 1

Brand : Servicebio

Share

Product Information

Product Name

Cat. No.

Spec.

DMEM, High Glucose, powder

G4511P-1L

1 L

G4511P-10L

10 L

G4511P-50L

50 L

 

Product Description/Introduction

This product is powder DMEM high glucose medium, containing 4 mM L-alanyl-L-glutamine, 1 mM sodium pyruvate, phenol red indicator, without HEPES, additional sodium bicarbonate is required for use.

Storage and Shipping Conditions

Ship at room temperature; Store away from light at 2-8, valid for 3 years.

Assay Protocol / Procedures

Preparation

1. Prepare clean container close to final volume.

2. Prepare distilled water at room temperature (15-30°C) or water for cell injection (recommended G4701).

3. Prepare 1 N NaOH and 1 N HCl.

Steps to prepare solution with dry powder

1. Fill the prepared clean container with 95% final volume of distilled water, take 1 bottle of this product (can prepare 1 L of culture medium) or weigh the powder according to the proportion and put it into the water to dissolve. Note that the powder can be quickly dissolved after disintegration in water, such as high-speed stirring may make the powder into a mass, the formation of water film on the surface, but not conducive to dissolution. Therefore, the powder into the water without high-speed stirring.

2. After the powder is dissolved, add sodium bicarbonate according to the dosage of 3.7 g/L and stir to dissolve. If you need to prepare other volumes, add sodium bicarbonate according to the corresponding volume.

3. Adjust pH to 0.2-0.3 units below working pH with 1 N NaOH and 1 N HCl (recommended working pH 7.0-7.4). The solution will increase in pH by 0.1-0.3 units after filtration.

4. Add water to fix the volume to the final volume. Keep the solution sealed before filtration.

5. Filtration into sterile containers using 0.2 μm membranes (note sterile handling).

6. After filtration, a little liquid can be taken for aseptic inspection and used after passing. The prepared liquid medium is stored at 2-8 and valid for 1 years.

Note

1. Dry powder is easy to absorb moisture, seal in time after use.

2. Take care to protect yourself when taking dry powder to prevent inhalation.

3. Keep the medium container covered during stirring and filtering, and stop stirring when the dry powder is dissolved.

4. For your safety and health, please wear safety glasses, gloves and protective clothing.

 

Related Products

Cat. No.

Product Name

G4701

Pure water, laboratory use only

GC104004

Sodium bicarbonate

PET-500-YT-J

500 mL clear reagent bottle (sterile and enzyme-free with rounded shoulders)

PET-1000-YT-J

1000 mL clear reagent bottle (sterile and enzyme-free with rounded shoulders)

WG1MILLIPORE

Needle filter (0.2μm/PES/sterile)

For Research Use Only!

 

G4511P DMEM(High Glucose) Medium Formula

Components

Concentration


Components

Concentration

mg/L

mM

mg/L

mM

Amino acids




Inorganic salts



Glycine

30

0.4


Calcium Chloride (CaCl2) (anhyd.)

200

1.80

L-Arginine hydrochloride

84

0.4


Ferric nitrate nonahydrate ( Fe(NO3)39H2O)

0.1

0.0002

L-Cystine dihydrochloride

62.6

0.2


Magnesium Sulfate (MgSO4) (anhyd.)

97.67

0.80

L-Histidine hydrochloride-H2O

42

0.2


Potassium Chloride (KCl)

400

5.36

L-Isoleucine

105

0.8


Sodium Bicarbonate (NaHCO3)

3700

44.04

L-Leucine

105

0.80


Sodium Chloride (NaCl)

4750

81.2

L-Lysine hydrochloride

146

0.8


Sodium Phosphate monobasic (NaH2PO4) (anhyd.)

108.7

0.906

L-Methionine

30

0.2


Other components



L-Phenylalanine

66

0.4


D-Glucose (Dextrose)

4500

24.98

L-Serine

42

0.4


Sodium pyruvate

110

1.0

L-Threonine

95

0.8


HEPES

5958

25

L-Tryptophan

16

0.08


Phenol Red

14.95

0.0422

L-Tyrosine disodium salt dihydrate

103.79

0.4





L-Valine

94

0.8





L-Alanyl-L-Glutamine

868.9

4.0





Vitamins







Choline chloride

4.0

0.0286





D-Calcium pantothenate

4.0

0.0084





Folic Acid

4.0

0.0091





Niacinamide

4.0

0.0328





Pyridoxine hydrochloride

4.0

0.0195





Riboflavin

0.4

0.0011





Thiamine hydrochloride

4.0

0.0119





i-Inositol

7.2

0.0389





 

https://www.servicebio.com/goodsdetail?id=60385



This website uses cookies for best user experience, to find out more you can go to our Privacy Policy  and  Cookies Policy