DMEM, high glucose, GlutaPlus, HEPES, no sodium pyruvate, 500 mL
Attribute:
SKU : G4512-500ML
Categories : 2. Cell & Molecular Biology , Cell Culture Medium / Sera , Servicebio ,
Brand : Servicebio
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Product Information
Product Name | Cat. No. | Spec. |
DMEM, high glucose, GlutaPlus, HEPES, no Sodium Pyruvate | G4512-500ML | 500 mL |
Product Description
Dulbecco's Modified Eagle Medium (DMEM), is a modified medium based on MEM, is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12.
This product contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture, without protein or growth factors. Need to add 5-10% serum or serum-free additives depending on the cell type.
This product is sterilized by 0.1μm filter membrane, pH 7.0-7.4, contains 25 mM D-glucose, 4.0 mM GlutaPlus, phenol red indicator and HEPES buffer system, no sodium pyruvate.
GlutaPlus, L-alanyl-L-glutamine, is a cell culture additive that replaces medium moles of L-glutamine in cell culture media. The substance is stable in aqueous solution and does not degrade spontaneously. Instead, it is slowly degraded by a peptidase secreted by cells to release glutamine, which is then taken up and used by cells.
HEPES is an excellent biological buffering agent with no toxic effect on cells. The medium supplemented with HEPES can maintain a constant pH range for a long time.
Storage and Shipping Conditions
Ship at room temperature; Store away from light at 2-8, valid for 12 months.
Note
1. This product has been filtered and sterilized. Attention should be paid to aseptic operation to avoid contamination.
2. Avoid freezing the culture medium. Freezing may cause precipitation and precipitation of nutrients, which may not be completely dissolved after rewarming, affecting the cell culture effect. In addition, when the medium is stored at 2-8, do not store it completely close to the inner wall of the refrigerator to prevent the medium from freezing due to localized low temperature.
3. For your safety and health, please wear safety glasses, gloves and protective clothing.
For Research Use Only!
G4512 DMEM (High Glucose) Medium Formula
Components | Concentration | Components | Concentration | |||
mg/L | mM | mg/L | mM | |||
Amino acids | Inorganic salts | |||||
Glycine | 30 | 0.4 | Calcium Chloride (CaCl2) (anhyd.) | 200 | 1.80 | |
L-Arginine hydrochloride | 84 | 0.4 | Ferric nitrate nonahydrate ( Fe(NO3)39H2O) | 0.1 | 0.0002 | |
L-Cystine dihydrochloride | 62.6 | 0.2 | Magnesium Sulfate (MgSO4) (anhyd.) | 97.67 | 0.80 | |
L-Histidine hydrochloride-H2O | 42 | 0.2 | Potassium Chloride (KCl) | 400 | 5.36 | |
L-Isoleucine | 105 | 0.8 | Sodium Bicarbonate (NaHCO3) | 3700 | 44.04 | |
L-Leucine | 105 | 0.80 | Sodium Chloride (NaCl) | 4750 | 81.9 | |
L-Lysine hydrochloride | 146 | 0.8 | Sodium Phosphate monobasic (NaH2PO4) (anhyd.) | 108.7 | 0.906 | |
L-Methionine | 30 | 0.2 | Other components | |||
L-Phenylalanine | 66 | 0.4 | D-Glucose (Dextrose) | 4500 | 24.98 | |
L-Serine | 42 | 0.4 | Sodium pyruvate | |||
L-Threonine | 95 | 0.8 | HEPES | 5958 | 25 | |
L-Tryptophan | 16 | 0.08 | Phenol Red | 14.95 | 0.0422 | |
L-Tyrosine disodium salt dihydrate | 103.79 | 0.4 | ||||
L-Valine | 94 | 0.8 | ||||
L-Glutamine | ||||||
L-Alanyl-L-Glutamine | 869 | 4.0 | ||||
Vitamins | ||||||
Choline chloride | 4.0 | 0.0286 | ||||
D-Calcium pantothenate | 4.0 | 0.0084 | ||||
Folic Acid | 4.0 | 0.0091 | ||||
Niacinamide | 4.0 | 0.0328 | ||||
Pyridoxine hydrochloride | 4.0 | 0.0195 | ||||
Riboflavin | 0.4 | 0.0011 | ||||
Thiamine hydrochloride | 4.0 | 0.0119 | ||||
i-Inositol | 7.2 | 0.0389 | ||||
