2X TOPsimple™ DyeMIX–nTaq,2X,1ml
Attribute:
SKU : P510T
Categories : 3. Protein & Enzyme , Enzynomics (Korea) ,
Brand : Enzynomics (South Korea)
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2X TOPsimple™ DyeMIX-nTaq is similarly formulated to the 2X TOPsimple™ PreMIX-nTaq except that it contains loading dyes for further convenience of use. Thus, the reaction mixtures after PCR cycles are ready for agarose gel electrophoresis. This product includes nTaq DNA polymerase (Cat. P025, P050), and is optimized for high efficiency PCR amplification with a high success rate.
Characteristics
- 2-Dye system: Easy for gel electrophoresis (xylene cyanol and Orange G)
- A-tail formation at 3’ ends of amplified duplex DNA
- Amplification of DNA fragments shorter than 3 kb (Suitable for general PCR analysis)
- Amplification of cDNA and genomic DNA.
- Primer extension
- Colony PCR
Components (2X)
- nTaq: 0.2 unit/μl
- nTaq buffer (containing 3 mM MgCl2)
- dNTP mixture: 0.4 mM each
- Stabilizer
- Dyes: Xylene cyanol and Orange G
Migration of dyes in agarose gel
In an ordinary agarose gel, xylene cyanol co-migrate with 4-kb DNA fragments, and Orange G with 50-bp DNA fragments.
Storage
Stable up to 18 months at -20℃ or 3 months at 4℃ (Storage at -20℃ is recommended).
https://www.enzynomics.com/shop/product_item.php?it_id=201003
