BioFact™ RT-Kit (M-MLV, RNase H-), RTase,Reaction Buffer (include each 10mM dNTP), 8 mM DTT, 10000U (200U/ul)

BioFact™ RT Series is a complete system for synthesis of cDNA from RNA which is using BioFact™ RTase, a recombinant type of reverse transcriptase from M-MLV. Existing wild type of reverse transcriptase may have lower efficiency of cDNA synthesis due to its activation temperature at 42°C or below, however this mutant form has greater efficiency as inhibition of RNA's secondary structure by its thermostable characteristic at 50°C for activation. Therefore, BioFact™ RT Series is supplied successful system to synthesis of 1st-stranded cDNA up to 14kb from total RNA.

• Recombinant type from M-MLV
  
• cDNA synthesis with high processivity and sensitivity
  
• Optimal activity at 50 ℃(42-55℃) for great efficiency
  
• Synthesis of long length cDNA (<14kb ) from RNA

FIGURES (Q,S)

DETAILED INSTRUCTIONS

FEATURES

• RNase H : M-MLV RTase (RTase H-)
  
• Temperature of synthesis cDNA : 42 - 55 ℃
  
• Synthesis length :＜ 14 kb
• Synthesis primer : random primer, oligo-d(T),
  gene specific primer

APPLICATION

• First-stranded cDNA synthesis
  
• RT-PCR
• Real-Time PCR
  
• Primer extension analysis

STORAGE TEMPARATURE

• -20℃ (Stable for 7 days at 25℃)

EXPIRATION DATE

• 2 years and 3 months
  (in case of storage at -20℃)
  
• RNase Inhibitor : 1 years and 6 months
  (in case of storage at -20℃)

http://bio-ft.com/en/pro1_2_01