BioFact™ 2X Real-Time PCR Master Mix (final 2.5mM MgCl2), with Evagreen™,3 ml x 4ea

BioFact™ Real-time PCR Series is providing optimizing system for easy-use using intercalating reagent and probe in real-time PCR amplification. Included BioFact™ H-Star DNA Polymerase helps accurate and reliable result to enable target-specific amplification due to its hot start characteristic by inhibition of primer- dimer or non-specific binding. Optional or mixed fluorescent dye, EvaGreen™ is available to use as an intercalating reagent, even though high resolution melting (HRM) analysis is applicable for genetic analysis such as SNP genotyping, mutation discovery or CpG methylation. BioFact™ Real-time PCR Series which is for the purpose of Taqman® probe or other fluorescent dye such as SYBR® Green are also available with various easy-to-use types.

• Accurate detection of a wide range of template amounts
  
• Fast and accurate real-time quantification of target DNA
  
• High specific detection using H-Star Taq DNA polymerase

FIGURES (Q,C)

DEATAILED INSTRUCTIONS

Features of EvaGreen™ dye

• Spectral property : λabs / λem = 500 / 530 nm (DNA bound), λabs= 471 nm (w/o DNA)
  
• No PCR inhibition by dye concentration Very bright and stable fluorescence
• Equally binding to G+C rich and A+T rich regions
  
• Suitable for HRM analysis
  
• Non-mutagenic, non-cytotoxic
  
• Storage : -20℃ (light is blocked / No moisture)

APPLICATION

• DNA using real-time PCR
  
• Real-time quantification of RNA target
• High Resolution Melting (HRM) analysis

STORAGE TEMPARATURE

• -20℃ (Stable for 7 days at 25℃)
  

EXPIRATION DATE

• 1 Year (in case of storage at -20℃)

http://bio-ft.com/en/pro1_3_01