Polyacrylamide Gel (Page)

Product Information

Product Description/Introduction

This product is a kind of dye suitable for preparation of green polyacrylamide gel upper gels, the dye can be stable in the upper gels (stacking gels), will not enter the lower gels (separation gels) with electrophoresis. Therefore, the addition of the green dye can make the upper gels protein spotting wells clear, and it is easy to judge the margin of the top sample wells or whether they are distorted or broken, and facilitates the accurate addition of protein samples into the spotting wells. The dye will not affect the electrophoresis and staining effect, after the electrophoresis is completed, it is also easy to identify and remove the upper gel, does not affect the subsequent Western Blot and other experiments, can be used with the company's G2003, G2037 products.

Storage and Shipping Conditions

Ship at room temperature; Store at 2-8 or room temperature; Valid for 24 months.

Assay Protocol/Procedures

1. Due to the special nature of the dye, precipitation may occur during storage, so please mix upside down before use；

2. For a 1.0 mm gel, approximately 2 mL of upper gel solution is required to prepare a single gel, which can be added at a ratio of 2 μL of upper green dye (500 x) per 1 mL of upper gel solution and mixed well;

3. Add the upper gel polymerisation catalyst (such as ammonium persulphate or other ammonium persulphate substitutes) and TEMED, mix well and inject the upper gel solution between the gel plates using a pipette and insert into the electrophoresis comb;

4. Remove the comb for subsequent electrophoresis after the upper gel is solidified.

Note

1. The dye may also be added after the addition of a gel polymerization catalyst (such as ammonium persulfate or other ammonium persulfate substitutes) and TEMED, mixed and directly perfused with the upper gel.

2. Due to the phenomenon of parallel electrophoresis of multiple gels in different samples, the company also provides a variety of other colors of the upper gel dye, including green, yellow, blue to distinguish different samples from different gel electrophoresis.

3. It is normal for dyes to deposit or agglomerate, they can be dispersed by high-speed vortexing or ultrasonic treatment and then used. Seal the product promptly after use to prevent evaporation of the liquid.

4. For your safety and health, please wear safety glasses, gloves, or protective clothing.

For Research Use Only!