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หมวดหมู่ : Biochemicals ,  1. Chemical and Reagents ,  Buffers ,  Servicebio , 
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Product Information
Product Name | Cat. No. | Spec. |
Sodium Dodecylsulfate | GC204005-100g | 100 g |
GC204005-500g | 500 g |
Introduction
SDS, also known as sodium dodecyl sulfate, is easily soluble in water, but its solubility is sensitive to temperature and easy to precipitate from the solution at low temperature. It is a commonly used anionic surfactant, which can disintegrate the cell membrane, bind to the hydrophobic part of membrane protein and make it separate from the membrane. High concentrations of SDS can also break non-covalent bonds such as ionic and hydrogen bonds in proteins and even change the conformation of proteins. This property is often used in SDS gel electrophoresis for protein composition analysis, in which 10%SDS is often used as a reserve solution. In addition, SDS can rapidly destroy tissues and inhibit RNase and DNase activities, so SDS is also a key component in nucleic acid purification reagents.
Basic Attributes
Name | Sodium Dodecylsulfate |
Synonym | Sodium lauryl sulfate; sodium lauryl sulfate; dodecyl sodium sulfate |
CAS | 151-21-3 |
Molecular formula | C12H25O4SNa |
Molecular mass | 288.38 |
Purity | 95% |
Appearance (character) | White to white powder or particles |
Storage conditions | Room temperature |
Unit | Bottle |
Spec. | 100 g / 500 g |
Pubchem CID | 3423265 |
MDL No. | MFCD00036175 |
EC No. | 205-788-1 |
Related categories | Organics, surfactant |
Solubility | ca. 150 g/L (20) |
Validity | 36 months |
Storage and Handling Conditions
Transport at room temperature, store at room temperature.
Instruction
The usage and dosage of biochemical reagents are mainly based on the customer's experimental purpose and the experimental methods in the existing literature or books, and the following uses are for reference only.
1. Easily soluble in water, in WB experiments, used for gel making, the concentration is generally 10% SDS, in electrophoresis commonly used 10% SDS as a reserve solution; this product can be used in the pure water, laboratory use only (recommended G4701) or distilled water for dissolution, if the preparation of 10% SDS solution, can be weighed 100 g of SDS slowly transferred to a beaker containing about 0.9 L of water, stirring with a magnetic stirrer until completely Dissolve. Then, the solution should be diluted to 1 L with water. This procedure is for reference only and can be adjusted according to the experimental reagents.
2. Used in nucleic acid purification reagents, SDS rapidly destroys tissue and inhibits RNase and DNase activity. 10% or 20% SDS stock solution is usually used, and the working concentration of SDS is 0.1-0.5%.
Note:
1. Operate in a ventilated place with proper protection to avoid inhalation or direct contact with skin.
2. SDS is easily soluble in water, but its solubility is sensitive to temperature. SDS is easy to precipitate from the solution at low temperature, and can be used normally after rewarming and dissolution.
3. For your safety and health, please wear lab coat and disposable gloves when operating.
Security Information
WARNING | P261 |
RTECS | WT1050000 |
WGK | 2 |
Note: the product may be optimized and upgraded. The actual label information shall prevail.
For Research Use Only!