PMSF (100mM), 1 mL (for Protease Inhibitor)

Product Information

Product Description/Introduction

The synthesis and breakdown of proteins in cells are in dynamic balance under stable conditions. However, when extracting proteins from cells and tissues in vitro, cells contain many endogenous proteases that can degrade proteins, such as phosphatases, serine proteases, and so on. These ubiquitous endogenous proteases can lead to the decomposition of target proteins, thus affecting the experimental results. Protease inhibitors are used to protect proteins from degradation by endogenous proteases released during protein extraction. PMSF (Phenylmethanesulfonyl fluoride, also know as phenylmethylsulfonyl fluoride, molecular formula C7H7FO2S, molecular weight 174.19), can irreversibly inhibit serine protease (such as trypsin, chymotrypsin, thrombin) and thiol protease (such as papain).

Storage and Shipping Conditions

Ship with wet ice；Store at -20, valid for 12 months.

Product Components

Assay Protocol/Procedures

1. The concentration of this product is PMSF 100 mM, and the final concentration of working solution is recommended to be 1 mM.

2.  This product is added to protein lysate at a ratio of 1:100 about 5 minutes before use, and mix well for reserve use.

Note

1.  PMSF is highly toxic and seriously damages the mucosa of the respiratory tract, eyes and skin. It is fatal if inhaled, swallowed or absorbed through the skin. If PMSF comes into contact with eyes or skin, rinse immediately with plenty of water.

2. PMSF is unstable in aqueous solution and should be added to lysis buffer from storage solution before use.

3.  For your safety and health, please wear safety glasses, gloves, or protective clothing.

For Research Use Only!

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