DNA Polymerase I (E.coli), 500 U

Product Information

Product Introduction

DNA polymerase I ( E.coli ) is a DNA polymerase I derived from Escherichia coli and expressed by recombinant Escherichia coli. It retains the 5'-3' polymerase activity of DNA polymerase I. In the presence of template and primer (DNA or RNA), it uses dNTP as substrate to synthesize DNA complementary to the template in the 5'-3' direction. This enzyme also retains the double-strand-specific 5'-3' exonuclease activity and the single-strand-specific 3'-5' exonuclease activity.

Source: Derived from Escherichia coli and expressed by recombinant Escherichia coli.

Enzyme activity definition: The amount of enzyme required to catalyze the incorporation of 10 nmol of deoxyribonucleotide into polynucleotide within 30 min at 37°C is defined as one unit of enzyme activity.

Purity and concentration: Purity detected by SDS-PAGE 95%; residual endogenous nucleic acid <1 pg/μL (qPCR detection); 5 U/μL.

Inactivation or inhibition: Heating at 75 for 20 min or adding appropriate amount of EDTA can lead to enzyme inactivation. Metal ion chelators and inorganic pyrophosphates have an inhibitory effect on the enzyme.

Enzyme storage buffer: 25 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.4.

10х Reaction buffer: 100 mM Tris-HCl, 500 mM NaCl, 100 mM MgCl ₂ , 10 mM DTT, pH7.9.

Storage and transportation

Transported with wet ice; stored at -20, valid for 12 months.

composition

Precautions

1. The enzyme should be placed in an ice box when used and stored at -20 immediately after use.

2. For your safety and health, please wear a lab coat and disposable gloves when operating.

The product is for scientific research purposes only and not for clinical diagnosis!

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