Recombinant Mouse NCAM1 Protein (His-tag)

Gene Name Synonym

CD56, MSK39, NCAM1, N-CAM-1

Species

Mouse

Accession

P13595

Source

HEK293 cells

SPECIFICATIONS

Amino acid sequence

Leu20-Thr711 is expressed with a 10×His tag at the N terminus

Molecular Weight

78.5 kDa

Purity

>95.0% as determined by SDS-PAGE

Endotoxin

<5 EU·mg^-1

Formulation

Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

NCAM1 (Neural Cell Adhesion Molecule 1, also known as CD56) is a neural adhesion protein (NCAM) that belongs to the immunoglobulin superfamily.The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. NCAM1 is involved in neuron-neuron adhesion, neurite fasciculation, the outgrowth of neurites, etc. It has also been shown to be involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance.NCAM1 signaling is mediated either by homophilic or heterophilic interactions with fibroblast growth factor receptor (FGFR), L1-CAM, N-cadherin and other components of the extracellular matrix.Upon activation, NCAM1 triggers a variety of signaling cascades including FYNfocal adhesion kinase (FAK), MAPK, and phosphatidylinositol 3-kinase (PI3K) pathways.

Data

SDS-PAGE

Bioactivity

Active Recombinant Mouse NCAM1 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 80-140 kDa.

Immobilized Mouse NCAM1, His Tag at 10 μg/mL (100 μL/well) can bind Anti-NCAM1 with a linear range of 3.5-56.2 ng/mL.