Recombinant Human CD44 Protein (His-tag)

Gene Name Synonym

CD44, CDW44, CSPG8, ECMR-III, HCELL, HUTCH-I, IN,LHR, MC56, MDU2, MDU3, MIC4, Pgp1, Epican

Species

Human

Accession

P16070

Source

HEK293 cells

SPECIFICATIONS

Amino acid sequence

Gln21-Glu649 is expressed with a 10×His tag at the C terminus

Molecular Weight

69.1 kDa（The protein migrates as 100~150 kDa under reducing condition (SDS-PAGE) due to glycosylation）

Purity

>95.0% as determined by SDS-PAGE

Endotoxin

<5 EU mg^-1

Formulation

Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

CD44 is a type I transmembrane protein and a member of the cartilage link protein family. It is involved in cell-cell and cell-matrix interactions and signal transduction. CD44 is a ubiquitously expressed protein that is the major receptor for hyaluronan and exerts control over cell growth and migration. CD44-hyaluronate mediates in lymphocyte T and monocyte adhesion to the endothelium, stimulates proinflammatory cytokine release from macrophages and participates in dedifferentiation phenotype of smooth muscle cells from contractile state to synthetic one.CD44 protein is expressed on the majority of immune cells. Furthermore, an increasing quantity of evidence suggests that CD44 has various functions related to inflammatory disease.

Data

SDS-PAGE

Bioactivity

Active Recombinant Human CD44 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 90-120 kDa.

Immobilized Human CD44, His Tag at 10 μg/mL (100 μL/well) can bind Anti-CD44 with a linear range of 0.09-0.78 μg/mL.