Recombinant Human CD1A Protein (His-tag)

Gene Name Synonym

CD1A, CD1, FCB6, HTA1, R4, T6

Species

Human

Accession

P06126

Source

HEK293 cells

SPECIFICATIONS

Amino acid sequence

Asn17-Ser298 is expressed with a 10×His tag at the C terminus

Molecular Weight

33.5 kDa

Purity

>95.0% as determined by SDS-PAGE

Endotoxin

<5 EU mg^-1

Formulation

Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

The family of CD1 molecules consists of group 1 CD1 proteins (CD1a, CD1b, CD1c and CD1e) and group 2 CD1 proteins (CD1d). CD1a is a lipid-presenting molecule. CD1a is a transmembraneglycoproteins, which is structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. CD1a proteins are expressed in the skin at high density on the surface of Langerhans cells. Previous studies have shown the unique capacity of CD1a to exchange associated b2m with that present in the serum.

Data

SDS-PAGE

Bioactivity

Active Recombinant Human CD1A Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 35-40 kDa.

Immobilized Human CD1A, His Tag at 10 μg/mL (100 μL/well) can bind Anti-CD1A with a linear range of 0.97-31.2 ng/mL.