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หมวดหมู่ : 3. Protein & Enzyme ,  Recombinant protein ,  Servicebio , 
แบรนด์ : Servicebio
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DESCRIPTION | ||
Gene Name Synonym | CXCL8, GCP1, IL8, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1 | |
Species | Human | |
Accession | P10145 | |
Source | HEK293 cells | |
SPECIFICATIONS | ||
Amino acid sequence | Met23-Ser99 is expressed with a 10×His tag at the C terminus | |
Molecular Weight | 10.3 kDa | |
Purity | >95.0% as determined by SDS-PAGE | |
Endotoxin | <5 EU mg-1 | |
Formulation | Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization. | |
Reconstitution | Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation. | |
STORAGE | ||
Stability and Storage | 12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage. | |
BACKGROUND | ||
Interleukin 8 (IL8 or chemokine (C-X-C motif) ligand 8, CXCL8) is a chemokine produced by macrophages and other cell types such as epithelial cells, airway smooth muscle cells and endothelial cells. There are many receptors on the surface membrane capable of binding IL-8, the most frequently studied types are the G protein-coupled serpentine receptors CXCR1 and CXCR2. Expression and affinity for IL-8 differs between the two receptors (CXCR1 > CXCR2). | ||
Data | ||
SDS-PAGE | Bioactivity | |
Recombinant Human IL-8 Protein was determined by SDS-PAGE with Coomassie Blue. | Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC-T1). The ED50 for this effect is typically 1-5 μg/mL. |