Recombinant Human IL-8 Protein (His-tag)

Gene Name Synonym

CXCL8, GCP1, IL8, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1

Species

Human

Accession

P10145

Source

HEK293 cells

SPECIFICATIONS

Amino acid sequence

Met23-Ser99 is expressed with a 10×His tag at the C terminus

Molecular Weight

10.3 kDa

Purity

>95.0% as determined by SDS-PAGE

Endotoxin

<5 EU mg^-1

Formulation

Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

Interleukin 8 (IL8 or chemokine (C-X-C motif) ligand 8, CXCL8) is a chemokine produced by macrophages and other cell types such as epithelial cells, airway smooth muscle cells and endothelial cells. There are many receptors on the surface membrane capable of binding IL-8, the most frequently studied types are the G protein-coupled serpentine receptors CXCR1 and CXCR2. Expression and affinity for IL-8 differs between the two receptors (CXCR1 > CXCR2).

Data

SDS-PAGE

Bioactivity

Recombinant Human IL-8 Protein was determined by SDS-PAGE with Coomassie Blue.

Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC-T1). The ED50 for this effect is typically 1-5 μg/mL.