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หมวดหมู่ : 3. Protein & Enzyme ,  Recombinant protein ,  Servicebio , 
แบรนด์ : Servicebio
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DESCRIPTION | ||
Gene Name Synonym | IL-5, IL5, TRF, IL5, Interleukin-5 | |
Species | Human | |
Accession | P05113 | |
Source | HEK293 cells | |
SPECIFICATIONS | ||
Amino acid sequence | Asn49-Ser162 is expressed with a 10×His tag at the C terminus | |
Molecular Weight | 14.4 kDa | |
Purity | >95.0% as determined by SDS-PAGE | |
Endotoxin | <5 EU mg-1 | |
Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization. | |
Reconstitution | Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation. | |
STORAGE | ||
Stability and Storage | 12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage. | |
BACKGROUND | ||
IL-5 critically regulates expression of genes involved in cell survival, IgH switch recombination, maturation in B cells and genes required for growth, survival, and effector function of eosinophils. IL-5 was recognized as the major maturation and differentiation factor for eosinophils in mice and humans. IL-5 was originally defined as a T-cell-derived cytokine that triggers activated B cells for terminal differentiation into antibody-secreting plasma cells. IL-5 critically regulates expression of genes involved in proliferation, cell survival and maturation and effector functions of B cells and eosinophils. Growth in the number of circulating, airway tissue, and induced sputum eosinophils have been observed in patients with these diseases. Interleukin 5 has long been associated with several allergic diseases, including allergic rhinitis and asthma. | ||
Data | ||
SDS-PAGE | Bioactivity | |
Active Recombinant Human IL-5 Protein was determined by SDS-PAGE with Coomassie Blue. | Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.1-1 ng/mL. |
For Research Use Only!
Ver. No.: V1.0-202102