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หมวดหมู่ : 3. Protein & Enzyme ,  Recombinant protein ,  Servicebio , 
แบรนด์ : Servicebio
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DESCRIPTION | ||
Gene Name Synonym | IL-4, IL4, BCGF1, BSF1 | |
Species | Mouse | |
Accession | P07750 | |
Source | CHO cells | |
SPECIFICATIONS | ||
Amino acid sequence | His21-Ser140 is expressed with a 10×His tag at the C terminus | |
Molecular Weight | 15 kDa | |
Purity | >95.0% as determined by SDS-PAGE | |
Endotoxin | <5 EU mg-1 | |
Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization. | |
Reconstitution | Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation. | |
STORAGE | ||
Stability and Storage | 12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage. | |
BACKGROUND | ||
Interleukin-4, also known as IL4, is a secreted protein that belongs to the IL-4 / IL-13 family. IL-4 was first identified as a T cell-derived growth factor for B cells. In addition to T cells, IL-4 is produced by innate lymphocytes, such as NTK cells, and myeloid cells, such as basophils and mast cells. Interleukin-4 / IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation. Interleukin-4 / IL4 also regulates the expression of the low-affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Interleukin-4 is essential for the switching of B cells to IgE antibody production and the maturation of T helper (Th) cells toward the Th2 phenotype. It participates in at least several B-cell activation processes as well as other cell types. | ||
Data | ||
SDS-PAGE | Bioactivity 1 | |
Recombinant Mouse IL-4 Protein was determined by SDS-PAGE with Coomassie Blue. | Measured in a cell proliferation assay using mouse splenocytes. The ED50 for this effect is typically 0.1-0.5 μg/mL. |