Multi-functional Imaging System

Product Information

The SCG-W5000 is a comprehensive device that integrates chemiluminescence technology and gel imaging. it is

equipped with a high-sensitivity cooled camera with 9 million pixels, enabling rapid, accurate, and high

throughput detection and imaging of samples. it is widely used in the fields of life sciences, medicine, and

environmental protection.

Product Features

Chemiluminescence Imaging System :

1. Weak band signal, one-time imaging

2. Both strong and weak band signals can be achieved, and the desired results can be obtained

3. Source file can be saved, and the image can be adjusted at any time

4. High Efficient

Gel Imaging System:
Three modes and multiple parameters can be flexibly adjusted, resulting in clear and bright imaging
UV laser protective board, safe and convenient for gel cutting

TechnicalSpecifications

Notes

It is prohibited to touch or scratch the internal lenses of the dark box with hands or sharp objects

After placing the experimental samples, make sure to close the instrument's flip door to prevent external light from entering

the dark box and affecting the experimental results

During imaging experiments, shaking the experimental table or instrument is prohibited to avoid impacting the image

quality.

Pay attention to electrical safety. Pullingor moving the power cord during the experiment is prohibited

After the experiment is completed, clean the samples and any residues inside the dark box thoroughly

 Functional Description

Operating Procedures

Chemiluminescence Imaging Application

Power on

Plug in the power cord and turn on the power switch at the back of the instrument. The industrial computer will

start up.

Sample Loading

Open the instrument door, take out the sample tray, place the prepared text sample on the tray, and then place

the tray flat in the groove inside the instrument dark box.Closetheinstrumentdoor.

Launchinglmaging Software

After the industrial computer starts up, the application software will be automatically loaded. 0ncethe software

is successfully launched, it will navigate to the main page.

The top-left section displays the company logo.

The bottom-left section is the status bar, showing the current camera and control board connection status, as

well as detection of the inserted removable disk.

The top-right section displays the software version number.

The bottom-right section includes buttons for switching between simplified/traditional Chinese, switching to

English, exporting the page, and closing the program.

Clicking on the central icon will enter the preview and capture page

Clicking on the Chemiluminescence will enter the preview and capture page

Preview and Capture Page

On the preview page, the user needs to input the location for storing the experimental results. The file name is

optional and facilitates file retrieval for the user.

The user can choose between manual exposure and automatic exposure as the current experimental mode. 

For manual exposure, the user needs to input the exposure time, while for automatic exposure, the algorithm

calculates the optimal exposure time.

On the right side of the preview, the user can input a time value in microseconds (us). This time represents the

exposure time for the bright field image. Clicking the preview switch initiates the preview, and the preview time

can be adjusted as needed.

Clicking the capture button starts the exposure for capturing the image while clicking the return button takes

the user back to the main page.

Shooting Process

1. Select automatic exposure and set the preview time.

Automatic exposure intelligent exposure technology can quickly determine the optimal exposure time.

2. Real-time making

Click on the capture button to start the exposure. A strip is displayed on the left, and a countdown of the

exposure time is shown on the right. As the countdown progresses, real-time imaging of the sample signal

changes is displayed on the left.

Real-time imaging Presents the changes in the sample signal during the exposure process in real-time,

allowing users to grasp every detail of the capture. This breakthrough feature not only enhances shooting

efficiency but also greatly improves user interaction experience.

During real-time imaging, areas in the strip that are overexposed will be displayed in red. lf it is determined that

the strip meets the requirements, you can click on the stop button in the lower right corner to end the exposure

early.

3. Temporallmaging, Time Accumulation

After the exposure is complete, it automatically enters the results page, where adjustments can be made to the

captured results.

Temporal Imaging Through precise retrospective adjustment, users can select any frame within the exposure

time as the final output result.

Time Accumulation Even after the exposure is complete, users can choose to continue the exposure, allowing

the sample to receive additional exposure based on the already-exposed time. When clicking "Continue

Exposure," there is a prompt for the minimum exposure time. The set time needs to be greater than this minimum

time. lf the set time is shorter than this minimum time, the actual exposure time will be the minimum exposure

time indicated by the prompt.

Image adjustments, result saving

After obtaining satisfactory images through automatic exposure, continued exposure, and temporal imaging

adjustments can be made to the brightfield image, dark field image, and composite image on the results page.

Contrast Adjust the brightness and darkness of the bright field image

Exposure Adjust the lightness and darkness of the dark field image, Clicking on "auto-adapt" can recommend

the most suitable value

Rotation Rotate the image

Invert Apply an inverted color effect to the image, turning black into white and white into black

Crop Clicking on the crop will generate a region on the image. This region can be resized, and after selecting the

appropriate size clicking on crop again will crop out the desired area

Vertical flip Flips the image vertically

Horizontal flip Flips the image horizontally

Restore Restores the image to its original state

Im port Select the file automatically saved by the program to import it into the program for further operations

Image saving Save the image and also save the bright field image, dark field image, and composite image

Result saving Save the adjusted image as an encrypted file

If any adjustments are made to the image on the results page

Export Page

Storage location Select the location of the image files automatically saved by the system, and the contents of

the files will be displayed in a list format the system

Open file Open the selected directory

Export Selecttheexport contentandformat to exportthe images

Delete Select the image file to be deleted,and it will be deleted

Open  Select the corresponding file in the list to open and jump to the result page. You can operate on the image

on the result page, or double-click the corresponding file to open it

Return Click "return" to return to the main page

Gel Imaging Application

Power on

Plug in the power cord, turn on the power switch at the back of the instrument, and the industrial computer will

start up.

Sample Loading

Protein Gel Open the instrument door, take out the white sample tray, place the prepared sample to be tested

on the tray, then place the tray flat in the groove inside the instrument dark box, and close the instrument door.

Nucleic Acid Gel Open the instrument door, place the sample on the ultraviolet transmissive glass of the

ultraviolet light source module, with markings on the surface of the ultraviolet glass indicating the range for

placing the sample.

Load Application software

After the industrial computer starts up, the application software is automatically loaded. Once the software

starts up normally, it will navigate to the main page.

The upper left part displays the company;

The lower left part is the status bar, showing the connection status of the current camera and control board, as

well as the detection of inserted removable disks;

The upper right part displays the software version number;

The lower right part includes buttons for switching between simplified and traditional Chinese, switching to

English, accessing the export page, and closing the program;

Clicking on the icon in the middle and selecting, "Gel lmaging" will take you to the preview and capture page.

 Preview and Capture Page

Preview and capture pages include status display information, camera settings, lens settings, mode settings,

image saving, and cropping, etc.

The top bar consists of a light source status indicator, keyboard open button, minimize, window restore, exit gel

imaging; file path input to save the location of the image, file name input to save the name of the image.

The middle window displays real-time captured images from the camera.

The bottom bar includes camera/control board/mobile disk connection status display information, image

format selection for saving (tiff/jpeg/bmp), save image button, open image path, and image cropping button.

The right is for camera settings, mode settings, and lens settings, which are described as follows:

Video settings Double-click" Video Settings" to center the image

Contrast Adjusts brightness, range -100~100, default value is 0, increasing the value makes the image brighter,

decreasing the value makes it darker

Exposure Sets the exposure time in milliseconds, default time is 350ms, increasing the time makes the image

brighter

Gain Sets the gain value, default value is 500%, range 100%~5000%, increasing the gain makes the image

brighter

Auto Exposure In Automatic exposure mode, the camera automatically adjusts exposure time and gain

Manual Exposure Manual exposure mode, manually set exposure time and gain

Negative Film Inverts blackand white colors

lllumination Turnson/off the illumination LED, used for capturing protein gel mode

UV Light Turns on/off the UV light source, can only be operated when the door is closed, used for capturing

nucleic acid gel mode

Gel cutting, Turns on/off the gel cutting mode, and can only be operated when the door is open, when gel cutting is

turned on, the UV light source will also automatically turn on, UV protective plate must be placed before opening

Adjustment Mode Adiuststhefocusand aperture range of the motorized lens

Coarse Adjustment Large adjustment step size, focusing adjustment step size of400, aperture adjustment step

size of 200, when the image is completely blurred, coarse adjustment can quickly bring the image to a relatively

clear state

Fine Adjustment Medium adjustment step size, focusing adjustment step size of 200, aperture adjustment step

size of40,after coarse adjustment,fine adjustment can make the image clearer

Ultra-fine Adjustment Small adjustment step size, focusing adjustment step size of 15, aperture adjustment

step size of 15 can bring the image to the clearest state

Focus Adjusts the focal length range (0-4500) through the "." and "+" buttons, the value below indicates the focus

motor adjustment step range and real-time step

Aperture Adjusts the aperture range (0-2150) through the "." and "+" buttons, the value below indicates the

aperture motor adjustment strange and real-time step

Shooting Process

1. Select auto exposure, default contrast is 0, default exposure is 350, default gain is 500.

2. Nucleic Acid Gel: Turn on UV light, open the UV light source, then the bands can be seen in the window

Protein Gel: Turn on illumination, open the bright field light source, then the bands can be seen in the window.

3. Adjust the focus to make the bands as clear as possible

4. The default aperture step is 1700, which can be adjusted as needed. It is not recommended to adjust it too large

or too small. It is recommended to adjust it in the range of 1500-1900. The aperture is mainly used to adjust the

brightness of the bands, lf adjusting the aperture does not achieve the desired brightness of the bands, switch to

manual exposure and set the exposure time, which can significantly change the brightness.

5. Enter the file path and filename, then click"Save Image" to save the current image.

If only certain bands are needed, click on "image Crop" to enter the image cropping page. Slide the screen from

the upper left corner of the desired band to its lower right corner to create a red box outlining the band. Double.

clicking on any position within the red box will enlarge the band to fill the entire window.

Horizontal and vertical flips can be used to flip the bands along the horizontal and vertical directions, and

'Restore" can return the bands to their original state before flipping.

Once the bands are adjusted to the desired state, enter the file path and filename.

Click "Save image" to save the current image. Click"Exit" to return to the preview and shooting page.

 Gel cutting

1.0pen the instrument door, and the UV light source will automatically turn off.

2. Pull out the drawer containing the UV light source and place the UV protective shield diagonally on the drawer

and the instrument to block the UV light source from direct exposure to the eves.

Click on "Cutting Gel," the UV light source will turn on, and at this point, the bands of nucleic acid gel can be

observed, and the gel cutting action can be carried out.