Gel Imaging System

Introduction

SCG-W1000 is a device for imaging gels, equipped with a high-sensitivity 6.3-megapixel camera for rapid, accurate, and high-throughput detection and imaging of samples. It is widely used in the fields of life sciences, medicine, and environmental protection.

Product Features

Three modes and multiple parameters can be flexibly adjusted, resulting in clear and bright imaging;
UV laser protective plate, ensuring both safety and convenience in gel cutting.

Technical Specifications and Precautions

Operating Procedures

Power On

Plug in the power cord and turn on the power switch at the back of the instrument. The industrial computer will start up.

Sample Loading

Protein Gel: Open the instrument door, take out the white sample tray, place the prepared text sample on the tray, and then place the tray flat in the groove inside the instrument dark box. Close the instrument door.

Nucleic acid gel: Open the instrument door, place the sample on the UV-transmitting glass of the UV light source module. There are markings on the UV glass surface indicating the sample placement area.

Launching the Imaging Software

Preview and Capture Pages

The preview and capture page includes status display information,  camera settings, lens settings,  mode settings, image saving and cropping, etc.

The top bar includes UV light source status indicator, keyboard open button, minimize, window restore, exit gel imaging; file path input for saving the location of the image, file name input for saving the name of the image.

The middle window displays real-time images taken by the camera.

The bottom bar includes camera/control board/mobile disk connection status display information,  image format selection for saving (TIFF/JPEG/BMP), save image button, open image path, image crop button.

The right sidebar is for camera settings, mode settings, and lens settings area, details are as follows:

Video Settings Double-clicking "Video Setting" will center the image

Contrast    Brightness  adjustment,  range  -100~ 100,  default value  0,  increasing  the value  makes  the  image brighter, decreasing the value makes the image darker

Exposure Set exposure time in milliseconds, default time 350ms, increasing the time makes the image brighter Gain Set gain value, default value 500%, range 100%~5000%, increasing the gain makes the image brighter

Auto Exposure Automatic exposure mode, the camera automatically adjusts exposure time and gain Manual Exposure Manual exposure mode, manually set exposure time and gain

Inverse Color Black and white color inversion

Lighting Turn on/off the LED lighting, used for shooting protein gel mode

UV Light  Turn on/off the UV light source, can only operate on/off when the door is closed, used for shooting nucleic acid gel mode

Gel Cutting Turn on/off the gel cutting mode, can only operate on/off when the door is open, when opening gel cutting, the UV light source will also automatically turn on, used for cutting gel, the UV protection shield must be placed before opening gel cutting

Adjustment Mode Select the zoom, focus, and aperture adjustment range of the electric lens

Coarse Adjustment   Larger adjustment steps, zoom adjustment step 200, focus adjustment step 1000, aperture adjustment step 200, when the image is completely blurry, coarse adjustment can quickly make the image relatively clear

Fine Adjustment  Moderate adjustment steps, zoom adjustment step 50, focus adjustment step 250, aperture adjustment step 50, after coarse adjustment, fine adjustment can make the image clearer

Super Fine Adjustment  Smaller adjustment steps, zoom adjustment step 5, focus adjustment step 25, aperture adjustment step 5, can make the image in the clearest state

Zoom  Increase and decrease field of view by clicking "-" and "+" buttons Focus Adjust image clarity by "-" and "+" buttons

Aperture Adjust image brightness by "-" and "+" buttons

Capturing Process

1.Select automatic exposure, default contrast is 0, default exposure is 350, and default gain is 500 .

2. For nucleic acid gel: Click on the UV light, turn on the UV light source, then the bands can be seen in the window.

For protein gel: Click on the illumination, turn on the bright field light source, then the bands can be seen in the window.

3.Adjust the "zoom" to fill the field of view with bands, or adjust to the experimenter 's desired field of view.

4.Adjust the "aperture," first adjust to the maximum, then click "  " twice for fine adjustment, and the aperture is set to 3 .

5.Adjust the "focus " to make the image as clear as possible; in rare cases, if the results of fine adjustment and super-fine adjustment are still  not clear enough, adjust to  reduce the  "aperture," then focus to obtain the clearest image.

6.Enter the file path and file name, click "Save Image" to save the current image.

6. If only part of the bands is needed, click on " image crop" to enter the image cropping page. Slide the screen from the upper left corner of the desired band to its lower right corner, and a red box will appear to outline the band. Double-click on any position inside the red box to zoom in the band to fill the entire window; Horizontal flip and vertical flip can be used to flip the band along the horizontal and vertical directions, while restore can return the band to its original state before flipping; After adjusting the band to the desired state, enter the file path and file name, then click "Save Image " to save the current image; Click " Exit " to return to the preview and shooting page. If any adjustments are made to the image on the results page

Gel Cutting

1.Open the instrument door, the UV light source will automatically turn off.

2. Pull out the drawer of the UV light source, place the UV protective shield diagonally between the drawer and the instrument to shield the eyes from the UV light source.

3.Click on "Gel Cutting," the UV light source will turn on. At this point, the bands of the nucleic acid gel can be observed, and the gel cutting action can be performed.

Product Packing List

 https://www.servicebio.com/goodsdetail?id=72860