DNase Residue Detection Kit

DNase Residue Detection Kit

Product Information

Description/Introduction
Deoxyribonuclease (DNase) and ribonuclease (RNase) are a class of hydrolytic enzymes that degrade DNA and RNA molecules, which widely exist in the environment, reagents (such as enzymes) and biomaterials (such as PCR reaction tubes). The trace residue of nuclease can degrade a large number of nucleic acids, which has a certain impact on downstream biological experiments. Therefore, regular nuclease residue testing of reagents, consumables and containers in biological laboratories is necessary. 
The kit is a fluorescent probe-based high-sensitivity DNase rapid assay，and it contains a unique DNA modified substrate (DNase Substrate) with a fluorescence group and a quenching group at both ends, respectively. The excitation/emission wavelength of the fluorescence group is 575/602. Normally, because the distance between the fluorescence group and the quenching group is relatively close, the substrate can only emit weak fluorescence. In the presence of DNase, the bond between the fluorescence group and the quenching group is cut off, and a large number of fluorescence signals can be produced under the excitation light wave, only 30 min is needed for the whole detection process of the kit. Since the fluorescence of DNA substrate increases with the increase of DNase activity, the results monitored by enzyme-labeled instrument can be Kinetically evaluated.
The kit provides DNaseⅠas the positive control of the detection system, and the minimum detection amount of DNaseⅠis 5×10-4 U. In addition to DNaseⅠ, it can also detect a variety of other DNase, such as Benzonase, Micrococcal Nuclease, Mung Bean Nuclease, S1 Nuclease, Exonuclease Ⅲ, etc.

Storage and Handling Conditions
Ship with wet ice and store at -20°C, valid for 12 months.