RNase Residue Detection Kit

RNase Residue Detection Kit

Product Information

Description/Introduction
Ribonuclease (RNase) is a kind of RNA hydrolase, which generally exists in the environment, and has high concentration residues in some biomaterials, enzymes and reagents, which has a very adverse effect on the related experimental operation of RNA. Therefore, it is necessary to detect the residue of RNA in any solution or biomaterial that comes into contact with RNase.
The kit contains a unique RNA modified substrate (RNase Substrate), which can quickly and sensitively detect the activity of RNase. The RNA modified substrate has a fluorescence group at one end and a quenching group at the other end. When there is no RNase, the quenching group is close to the fluorescence group, which suppresses the fluorescence of the fluorescence group to a very low level. In the presence of RNase, the RNA modified substrate is hydrolyzed, the fluorescence group and quenching group are separated in space, and the fluorescence group produces a large number of fluorescence signals. Since the fluorescence of RNA substrate increases with the increase of RNase activity, the results monitored by enzyme-labeled instrument can be Kinetically evaluated.
The kit provides RNase A as the positive control of the detection system, and can detect RNase A as low as 0.5 pg. In addition to detecting the activity of RNase A, it can also detect the activity of RNase T1, Micrococcal Nuclease, S1 Nuclease, Mung Bean Nuclease and Benzonase, etc. In addition, the kit is compatible with the DNase detection system. Because RNA substrate and DNA substrate have two different fluorescence labels, two kinds of substrates can be added to the same reaction system, and real-time analysis of RNase and DNase in a single sample can be carried out by enzyme-labeled instrument.

Storage and Handling Conditions
Shipped with wet ice and stored at -20°C. valid for up to 1 year.