nTaq-HOT (UDG plus),5units/μl,250U
คุณสมบัติสินค้า:
SKU : P725U
หมวดหมู่ : 3. Protein & Enzyme , Enzynomics (Korea) ,
แบรนด์ : Enzynomics (South Korea)
Share
nTaq-HOT (UDG plus) is a hot start PCR polymerase which remains inactive at temperatures lower than 75℃. Nonspecific elongation of incorrectly annealed primers before initiation of PCR reaction is one major cause of nonspecific DNA amplification. For this reason, amplification of nonspecific bands can be effectively prevented by using nTaq-HOT, thus increasing the specificity of target DNA amplification. Also, UDG and dUTP are included in the mixture to prevent the reamplification of carry-over PCR products between reactions.
Characteristics
- Molecular weight: 94 kDa
- Error rate: 2.4 X 10-5
- Thermal stability: half life of 40 min at 95℃
- A-tail formation at 3’ ends of amplified duplex DNA.
- No activity at temperatures lower than 75℃. Heating up to 95℃ results in activation of enzyme.
Applications
- High specific amplification of DNA fragments shorter than 3kb.
- Amplification of cDNA and genomic DNA.
- Amplification of template DNA with secondary or higher ordered structure that is resistant to PCR amplification
- Well-suited for an automated PCR machines, for which PCR reaction mixtures are prepared at room temperature
- Primer extension
Supplied with
- 10X nTaq-HOT buffer
- dNTP Mixture with dUTP (2 mM each)
- Sterile water
Quality control
- Purity: >99% on SDS-PAGE
- Endonuclease-free
- Exonuclease-free
- RNase free
- Inhibitor-free
Unit definition
One unit is defined as the amount of enzyme required to incorporate 10 nmol of dNTP into acid insoluble materials in 30 min at 74℃ in a 50-μl reaction mixture (20 mM Tris-HCl/pH 8.8, 50 mM KCl, 2.5 mM MgCl2, 10 mM β-mercaptoethanol, 12.5 μg of calf thymus DNA).
10X nTaq-HOT buffer
Containing 15 mM MgCl2
Cautions
nTaq-HOT (UDG plus) requires 10 minutes of initial denaturation to ensure effective activation of the enzyme.
https://www.enzynomics.com/shop/product_item.php?it_id=203007
