Magnetic Beads Viral DNA/RNA Extraction Kit II

Magnetic Beads DNA/RNA Extraction/Magnetic Beads Virus DNA/RNA KitThe Magnetic Beads Virus DNA/RNA Extraction Kit was designed specifically for efficient purification of viral DNA and viral RNA from cell-free samples such as serum, plasma, body fluids, nasopharyngeal and oropharyngeal swabs in viral transport medium (VTM) and the supernatant of viral infected cell cultures. Viral DNA/RNA is bound to the surface of the magnetic beads and released using a proprietary buffer system. The Magnetic Beads Viral DNA/RNA Kit can be easily adapted to automated magnetic bead separation instruments and workstations. The purified DNA/RNA can be used in qPCR and qRT-PCR assays.

DESCRIPTION
Specifications (Cat. # MV048, MV096)

• High Sensitivity: virus RNA/DNA can be successfully extracted and detected from as low as 10E1 copy number!
  
• Easily adapted to automated magnetic bead separation instruments and workstations
  
• Magnetic Bead Concentration: 50 mg/ml
  
• Magnetic Bead Size: ~ 5 µm
  
• Sample: up to 200 µl of virus samples (plasma, serum, body fluid or nasopharyngeal and oropharyngeal swabs in viral transport medium (VTM) 
  
• Manual or automated DNA/RNA isolation
  
• Operation time: within 30 minutes (manual) 
  
• Storage: dry at room temperature (15-25ºC)

APPLICATIONS
RT-PCR/PCR, qPCR, qRT-PCR, Real-time PCR, Real-time RT-PCR, Automated Fluorescent DNA Sequencing, Next Generation Sequencing (NGS)

COMPONENTS

• MV1 Buffer
  
• W1 Buffer*
  
• Wash Buffer*1
  
• RNase-free Water
  
• MV Magnetic Beads
  
• Carrier RNA2
  
• 96 Deep Well Plate
  
• Adhesive Film

QUALITY CONTROL
The quality of the The Magnetic Beads Virus DNA/RNA Extraction Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system by isolating viral DNA/RNA from a 200 µl serum sample.
Magnetic Beads Virus DNA/RNA Extraction Kit Functional Test Data

Figure 1 .Virus RNA was purified from 10E1-10E4 copy number of Red Spotted Grouper Nervous Necrosis Virus (RGNNV) using the Geneaid Magnetic Beads Virus DNA/RNA Extraction Kit (2 replications of each copy number). The purified RNA was eluted with 50 μl RNase-free Water. cDNA synthesis was carried out with a 10 μl aliquot of purified RNA using a Transcriptor First Strand cDNA Synthesis Kit (Roche) in a final volume of 20 μl. A Real-time PCR assay was then performed with 5 μl of synthesized cDNA as template, primers (designed to amplify the T4 region on the RNA2 segment), and Fast SYBR Green PCR Master Mix using the StepOnePlusTM Real-Time PCR system (Applied Biosystems). The results confirmed that virus RNA can be successfully extracted and detected from as low as 10E1 copy number of RGNNV.

DOCUMENTS

Magnetic Beads Virus DNA/RNA Extraction Kit II

https://geneaid.com/Magnetic-Beads-Virus-Extraction/MV