RNA Extraction/Total RNAThe Presto™ Mini RNA Yeast Kit was designed for total RNA purification from yeast and a wide variety of other fungus species using an efficient RNA miniprep system. This RNA Kit includes Sorbitol Buffer to reduce sample preparation time and minimize hands on time. The Presto™ Mini RNA Yeast Kit uses detergents and chaotropic salt to lyse cells and inactivate RNase. RNA in the chaotropic salt is bound by the glass fiber matrix of the spin column and once any contaminants have been removed, using the Wash Buffer, the purified total RNA is eluted by RNase-free Water. High quality total RNA can be purified within 20 minutes and is ready for use in RT-PCR, Northern Blotting, Primer Extension, mRNA Selection and cDNA Synthesis. Each Geneaid RNA Kit includes individually packaged certified DNase and RNase-free spin columns and collection tubes and DNase I for DNA sensitive downstream applications. DESCRIPTION Specifications (Cat. # RBYD050, RBYD100, RBYD300) Purify total RNA within 20 minutes!
High Yield: 5-30 μg of pure RNA
Convenient: Includes premixed Sorbitol Buffer
Sample: up to 5 x 107 yeast or other fungus species
Spin Columns: glass fiber membrane optimized for total RNA extraction
Individually packaged RNA spin columns and collection tubes, certified RNase and DNase-free
Storage: dry at room temperature (15-25°C), DNase I is shipped at room temperature and should be stored at -20°C for extended periods APPLICATIONS RT-PCR, Northern Blotting, Primer Extension, mRNA Selection, cDNA Synthesis COMPONENTS Sorbitol Buffer
RB Buffer
DNase I
DNase I Reaction Buffer
W1 Buffer
Wash Buffer
RNase-free Water
RB Columns
2 ml Collection Tubes QUALITY CONTROL The quality of the Presto™ Mini RNA Yeast Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. The purified RNA (A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and analyzed by electrophoresis. Presto™ Mini RNA Yeast Kit Functional Test Data
Figure 1 .Total RNA was extracted using the Presto™ Mini RNA Yeast Kit. Saccharomyces cerevisiae (5×107) was harvested by centrifugation at 5,000 x g for 10 minutes. A 5 µl aliquot of purified RNA from a 50 µl eluate was analyzed by electrophoresis on a 0.8% agarose gel.