Total RNA Mini Kit (Plant) w/DNase Set

RNA Extraction/Total RNAThe Total RNA Mini Kit (Plant) is a RNA Isolation Kit designed for total RNA purification from plant tissue and cells using an efficient RNA miniprep system. This RNA Kit will purify RNA from a wide variety of plant species within 15 minutes. Samples are initially ground in liquid nitrogen and then filtered to remove cell debris using the efficient RNA filter columns. In the presence of a binding buffer and chaotropic salt, total RNA in the lysate binds to the glass fiber matrix of the RNA spin column. Once any contaminants have been removed using the Wash Buffer (containing ethanol), the purified total RNA is eluted by RNase-free Water. Phenol extraction or alcohol precipitation is not required and the purified total RNA is ready for use in RT-PCR, Real-time PCR and Northern Blotting. Each Geneaid RNA Kit includes individually packaged certified DNase and RNase-free spin columns and collection tubes and DNase I for DNA sensitive downstream applications.

DESCRIPTION
Specifications (Cat. # RPD050, RPD100, RPD300)

• Purify total RNA within 15 minutes!
  
• High Yield: 5-30 μg of pure RNA
  
• Sample Size: up to 100 mg (fresh plant tissue), up to 25 mg (dry plant tissue)
  
• Spin Columns: glass fiber membrane optimized for total RNA extraction
  
• Individually packaged RNA spin columns and collection tubes, certified RNase and DNase-free
  
• Storage: dry at room temperature (15-25°C), DNase I is shipped at room temperature and should be stored at -20°C

APPLICATIONS
RT-PCR, Northern Blotting, Real-time PCR

COMPONENTS

• RB Buffer
  
• PRB Buffer
  
• DNase I
  
• DNase I Reaction Buffer
  
• W1 Buffer
  
• Wash Buffer
  
• RNase-free Water
  
• Filter Columns
  
• RB Columns
  
• 2 ml Collection Tubes

QUALITY CONTROL
The quality of the Total RNA Mini Kit (Plant) is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Total RNA from a 50 mg fresh Thale Cress (Arabidopsis thaliana) leaves is extracted using the Total RNA Mini Kit (Plant). The purified RNA (27 μg with an A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and checked by electrophoresis.

Total RNA Mini Kit (Plant) Functional Test Data

Figure 1 .Total RNA from 50 mg Thale Cress (Arabidopsis thaliana) leaves was extracted using the Total RNA Mini Kit (Plant). The purified RNA was analyzed by electrophoresis on a 1% agarose gel.

M = Geneaid 1 Kb DNA Ladder

Figure 2 .50 mg of Arabidopsis leaves RNA was purified using Geneaid Total RNA Mini Kit (Plant) Kit. The integrity and quality of RNA were determined by Qubit with RNA integrity and quality (IQ) assay kit. The IQ 8.8 score showed 88% large and structured RNA (including mRNA, tRNA and rRNA) was in the purified RNA.

DOCUMENTS

Total RNA Mini Kit (Plant)

https://www.geneaid.com/product.php?act=view&id=270