Presto™ 96 Well gDNA Bacteria Kit

DNA Extraction/Genomic DNA PurificationThe Presto™ 96 Well gDNA Bacteria Kit is optimized for high-throughput genomic and viral DNA purification from Gram (-) negative and Gram (+) positive bacterial cells. Gram+ Buffer, when combined with Lysozyme, will efficiently lyse bacterial cell walls consisting of the peptidoglycan layer. Proteinase K and chaotropic salt are used to further lyse cells and degrade protein, allowing DNA to easily bind to the glass fiber matrix of the binding plate. Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. Phenol/chloroform extraction or alcohol precipitation is not required and the purified genomic DNA is ready for use in a variety of downstream applications.

DESCRIPTION
Specifications (Cat. # 96GBB04, 96GBB10)

• High-throughput bacteria DNA extraction
  
• High Yield: up to 5 μg of genomic DNA per well
  
• High Purity gDNA: A260/A280 >1.9
  
• DNA Size: 20-40 kb
  
• Samples: Gram (+) Positive and Gram (-) Negative bacterial cells (up to 5 x 108)
  
• Convenient: Includes Gram+ Buffer for preparing Lysozyme solutions to speed up sample preparation when purifying DNA from Gram (+) positive bacteria
  
• 96 Well Binding Plates: glass fiber membrane optimized for genomic DNA extraction (sterilised to remove bacteria contamination)
  
• Elution volume: 100-200 µl
  
• Storage: dry at room temperature (15-25°C), Lysozyme is shipped at room temperature and should be stored at -20°C for extended periods

APPLICATIONS
PCR, Restriction Endonuclease Digestions, Array, Southern Blots and various other enzymatic reactions

COMPONENTS

• Proteinase K
  
• Gram+ Buffer
  
• GT Buffer
  
• GB Buffer
  
• W1 Buffer
  
• Wash Buffer
  
• Lysozyme
  
• Elution Buffer (10 mM Tris-HCl, pH8.5 at 25°C)
  
• Presto™ gDNA 96 Well Binding Plates
  
• Microtubes (Racked)
  
• Microtubes (8-strip)
  
• Caps for Microtubes (8-strip)
  
• 96 Deep Well Plates
  
• Adhesive Film

QUALITY CONTROL
The quality of the Presto™ 96 Well gDNA Bacteria Kit is tested on a lot-to-lot basis by isolating DNA from Escherichia coli (5×108) culture. 5 µl from a 100 µl eluate of purified DNA is analyzed by electrophoresis on a 1% agarose gel.

DOCUMENTS

Presto™ 96 Well gDNA Bacteria Kit

https://www.geneaid.com/product.php?act=view&id=190