Presto™ 96 Well Plant Genomic DNA Extraction Kit

DNA Extraction/Genomic DNA PurificationThe Presto™ 96 Well Plant Genomic DNA Extraction Kit is designed for high-throughput purification of total DNA (including genomic DNA, mitochondrial and chloroplast DNA) from various plant species. Homogenized samples are treated with RNase A then centrifuged to remove cell debris and salt precipitates. In the presence of a binding buffer, genomic DNA in the lysate binds to the glass fiber matrix of the Presto™ 96 Well gDNA Binding Plate. Contaminants are removed using a Wash Buffer and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. The entire procedure can be completed within 60 minutes without phenol/chloroform extraction or alcohol precipitation. The purified DNA (approximately 20-30 kb) is suitable for use in PCR or other enzymatic reactions.

DESCRIPTION
Specifications (Cat. # 96GPP04, 96GPP10)

• Purify 96 wells of pure genomic DNA from a variety of plant species in 60 minutes!
  
• Sample Volume: 100 mg of fresh plant tissue and 25 mg of dried plant tissue per well
  
• Yield: up to 30 μg of genomic DNA from 100 mg of fresh plant tissue per well
  
• Format: Presto™ 96 Well gDNA Binding Plates
  
• Elution Volume: 200~400 µl
  
• Centrifuge or vacuum manifold protocol
  
• Storage: dry at room temperature (15-25ºC)

APPLICATIONS
PCR, AFLP/PADP, RFLP, Southern Blotting, Real-time PCR

COMPONENTS

• GP1 Buffer and GPX1 Buffer
  
• GP2 Buffer
  
• GP3 Buffer
  
• RNase A
  
• W1 Buffer
  
• Wash Buffer
  
• Elution Buffer (10 mM Tris-HCl, pH8.5 at 25°C)
  
• Presto™ gDNA 96 Well Binding Plates
  
• 96 Deep Well Plates
  
• Microtubes (Racked)
  
• Microtubes (8-strip)
  
• Caps for Microtubes (8-strip)

QUALITY CONTROL
The quality of the Presto™ 96 Well Plant Genomic DNA Extraction Kit is tested on a lot-to-lot basis by purifying genomic DNA from corn leaf samples. The purified DNA is quantified with a spectrophotometer and analyzed by electrophoresis.

DOCUMENTS

Presto™ 96 Well Plant Genomic DNA Extraction Kit

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