Urine DNA Extraction Kit

DNA Extraction/Genomic DNA PurificationThe Presto™ Urine DNA Extraction Kit is designed for rapid isolation of high-quality cellular and cell-free DNA from up to 5 ml of urine. The kit includes uniquely designed Column Extension Tubes which allow for increased sample volume and yield. Urine samples are lysed using Proteinase K and a buffer containing chaotropic salt. The lysate is mixed with a binding buffer to facilitate DNA binding to the column membrane. The column is then washed and DNA is eluted with Elution Buffer. The entire procedure can be completed within 60 minutes and the purified DNA is ready for use in a variety of downstream applications such as qPCR, Next-Generation sequencing and DNA methylation analysis.

DESCRIPTION
SPECIFICATIONS

• Operation time: Purify cellular and cell-free DNA within 60 minutes.
  
• Sample: 1-5 ml of urine
  
• Format: Spin columns combined with column extension tubes using vacuum or centrifuge
  
• Yield: 1-20 ng of DNA per ml of male urine. 5-1000 ng of DNA per ml of female urine.
  
• Elution Volume: 30-50 µl
  
• Storage: dry at room temperature (20~25ºC)

APPLICATIONS
qPCR, Next-Generation sequencing and DNA methylation analysis

CAUTION
During operation, always wear a lab coat, disposable gloves, protective goggles and (anti-fog) procedure mask.

COMPONENTS

• UR1 Buffer
  
• UR2 Buffer
  
• Proteinase K
  
• W1 Buffer
  
• Wash Buffer
  
• Elution Buffer
  
• Carrier RNA
  
• Column Extender
  
• GD Column
  
• 2 ml Collection Tube

QUALITY CONTROL
The quality of the Presto™ Urine Extraction Kit is tested on a lot-to-lot basis by isolating DNA from 2 ml of urine. Following the purification process, the purified DNA integrity was assessed by qPCR.

DOCUMENTS

Presto™ Urine DNA Extraction Kit

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