GENEzol™ DNA Reagent Plant

Reagent DNA Extraction/Reagent DNA ExtractionGENEzol™ DNA Reagent Plant provides a quick and easy 3 step CTAB and chloroform based method to isolate total DNA (including genomic, mitochondrial and chloroplast DNA) from a variety of plant species (including algae and cyanobacteria). This unique reagent is able to lyse most common plant samples and plant samples with high a polysaccharide content. The extracted DNA is suitable for routine PCR screening, Real-Time PCR, Southern Blotting, Mapping and RFLP without phenol extraction.

DESCRIPTION
Specifications (Cat. # GR100, GR200)

• Extract high molecular weight genomic DNA from a variety of plant species
  
• Sample Volume: up to 1 g of fresh plant tissue and up to 0.5 g of dry plant tissue
  
• A cost effective, scalable solution
  
• Format: simple and gentle CTAB and chloroform based DNA precipitation method
  
• Storage: dry at room temperature (15-25ºC) for up to 2 years, RNase should be stored at 4ºC for extended periods

APPLICATIONS
PCR, Real-Time PCR, Southern Blotting, Mapping, RFLP

COMPONENTS

• GENEzol™ DNA Reagent Plant
  
• RNase A (50 mg/ml)

QUALITY CONTROL
GENEzol™ DNA Reagent Plant is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. 50 mg of fresh Arabidopsis leaves are initially ground in GENEzol™ DNA Reagent Plant. A 15 µl aliquot of extracted genomic DNA from a 100 µl eluate is analyzed by electrophoresis on a 1% agarose gel.

DOCUMENTS

GENEzol™ DNA Reagent Plant

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