Recombinant RNase A, 200 μL (for Nuclease/Topoisomerase)

Product Information

Product Description/Introduction

Recombinant RNase A is a Recombinant endoribonuclease that specifically target the 3' -ends of cytosine (C) or uracil (U) residues in single-stranded RNA and cleaves phosphate diester bonds with adjacent nucleotides. Recombinant RNase A has the highest cleavage activity for single stranded RNA. It is active under a wide range of reaction conditions. At low salt concentration (0-100 mM NaCl), it cleaves single stranded RNA, double stranded RNA, and RNA strand in RNA-DNA hybridization; at high salt concentration (300 mM NaCl), only single stranded RNA is specifically cleaved.

The Recombinant RNase A contains His-tag.

Applications:

To remove RNA from DNA preparations. minimize the risk of pathogen introduction into the bioprocess;

Other related research application in molecular biology and cell biology.

Feature: Specifically degraded RNA, purified by recombinant expression and free from contamination from animals, and rigorously control test for contaminating nonspecific endonuclease, exonuclease, and protease activity. 

Source: RNase A gene from Bovine pancreas, recombinant expression of Pichia pastoris.

Definition of enzyme activity: At room temperature, the amount of enzyme required to increase the A286 value by 0.0146 per minute in 1 mL reaction system. 

Enzyme activity is assayed in the following mixture: 100 mM Tris-acetate, 1 mM EDTA, 1 mM cyclic 2, 3-CMP, pH 6.5.

Purity and concentration:The purity is 95% by SDS-PAGE, DNase-free, Proteinase-free, 10 mg/mL.

Storage (Dilution) buffer: 50 mM Tris-HCl，50% Glycerol，pH 7.4 at 25.

Storage and Shipping Conditions

Ship with wet ice; Store at 20°C, valid for 12 months.

Product Contents

Note

1. To facilitate microdilution, dilute the Recombinant RNase A with Dilution Buffer as desired.

2. For your safty and health,please wear safety glasses, gloves, or protective clothing.