Recombinant DNase I (RNase-free)
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Categories : Biochemicals , 1. Chemical and Reagents , Buffers , Servicebio ,
Brand : Servicebio
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Product Information
Product Name | Cat.No. | Spec. |
Recombinant DNase I (RNase-free)
| G3342-500U | 500U |
G3342-10KU | 10KU |
Product Description/Introduction
DNase I is an endonuclease that hydrolyzes phosphodiester linkages yielding oligonucleotides with a 5'-phosphate and a 3'-hydroxyl group. DNase I is calcium-dependent and can be activated by magnesium and divalent manganese ions. In the presence of magnesium ion, DNase I could randomly splice any site of double-stranded DNA. In the presence of divalent manganese ions, DNase I can splice DNA double strands at the same site, forming blunt ends, or sticky ends with 1 or 2 nucleotides.
Applications:
1. Preparing RNA samples without DNA;
2. Destroy genomic DNA in RNA preparations prior to reverse transcription-PCR (RT-PCR).
3. In vitro T7, T3, SP6 and other RNA Polymerases catalyze the removal of DNA templates in the RNA post-transcription system;
4. Nick translationin DNA markers;
5. Generating libraries of random DNA fragments;
6. Apoptosis Tunel detection of partial splice of genomic DNA as positive control.
Features: specifically degrades DNA, but cannot degrade RNA.
Source: Recombinant expression of the pichia coli strain containing the Bovine Pancreatic DNase I gene.
Definition of enzyme activity: One unit is the amount of enzyme required for complete degradation of 1 µg pBR322 vector DNA in 10 minutes at 37.
Inactivation or inhibition: A 10-minute incubation at 75°C for complete inactivation of DNase I.
Purity: 95% by SDS-PAGE, RNase free; 10 U/uL.
Storage and Shipping Conditions
Ship with wet ice; Store at -20 valid for 12 months.
Product Contents
Component | G3342-500U |
Recombinant DNase I (RNase-free) | 50 μL |
10×DNase I Reaction Buffer | 1 mL |
25 mM EDTA | 1 mL |
Assay Protocol / Procedures
Reference use (e. g. prepare RNA samples without DNA)
a. Add the following component into a sterile, nuclease-free tube on ice in the indicated order, mix gently and centrifuge briefly.
Component | Amount |
RNA | 1 μg |
Recombinant DNase I (RNase-free) | 1 U |
10×DNase I Reaction Buffer | 1 μL |
RNase-free Water | to 10 μL |
1. Incubate at 37 for 30 minutes.
2. Add 0.5 μL of 25 mM EDTA to stop the reaction;
3. DNase I was completely inactivated after incubation at 75 for 10minutes.
Note
1. When using the product, the enzyme should be on ice and stored immediately after use at -20ºC.
1. For your safty and health,please wear safety glasses, gloves, or protective clothing.
For Research Use Only!
