GN3K DNA Marker

Product Information

Description/Introduction

The product GN3K DNA Marker consists of 8 linear double-stranded DNA bands (including 100 bp, 250 bp, 500 bp, 750 bp, 1000 bp, 1500 bp, 2000 bp, 3000 bp). The 1×DNA loading buffer mixed with blue indicator dye is suitable for the analysis of DNA bands in agarose gel electrophoresis. The product is ready-to-use, according to the experimental needs, directly take 5~10 μL for electrophoresis, and it is easy to use, electrophoresis band is clear, easy to accurately judge the content of the target product DNA. The DNA concentration of 500 bp band in GN3K DNA Marker is 100 ng/5 μL, which shows bright band, and the DNA concentration of other bands is 50 ng/5 μL.

Storage and Handling Conditions

Ship with wet ice and store at -20°C; valid for 24 months.

Product Contents

Assay Protocol / Procedures

1. After fully thawing and mixing, add 5 μL of the product to the sample hole of the agarose gel (the sample volume is proportional to the hole width: 1 μL: 1 mm, if the sample hole is wide, the sample volume can be increased appropriately) for electrophoresis.

2. The recommended electrophoretic condition is 1% agarose (recommended G5056) gel with a voltage of 4~10 V/cm.

3. Dye with EB or other nucleic acid dyes (recommended G3606) and observe the electrophoretic bands under ultraviolet light or gel imager.

Note

1. Thaw thoroughly before use and store at 4°C to avoid repeated freezing and thawing.

2. Recommended electrophoresis buffer: TAE/TBE (recommended G3001, G3002). Change the electrophoresis buffer in time and use the newly prepared agarose gel so as not to affect the electrophoresis results.

3. For your safety and health, please wear a lab coat and disposable gloves.

GN3K DNA Marker

1% TAE agarose gel stained by EB (sample size: 5 μL)

For Research Use Only!

https://www.servicebio.com/goodsdetail?id=6271