Special Fixative Solution for Fat
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Categories : Biochemicals , 1. Chemical and Reagents , Buffers , Servicebio ,
Brand : Servicebio
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Product Information
Product Name | Cat.No. | Spec. |
Special Fixative Solution for Fat | G1119-100ML | 100 mL |
G1119-250ML | 250 mL |
Description
In histopathology, tissues must be fixed in a timely and effective manner before they can be subsequently embedding and sectioning. The role of tissue fixation is to maintain the inherent form and structure of tissues and cells, prevent bacterial corrosion and autolysis of tissue cells, preserve the inherent substances in cells, coagulate intracellular proteins, minimize or terminate the reaction of endogenous and exogenous enzymes, so that cells or tissues basically remain the same as the substances in life, and also make tissues harden, which is convenient for tissue cutting. For some infectious specimens, it can prevent the spread of disease; Keep rough specimens. Finally, fixation enhances the effect of staining.
Adipose tissue contains a large number of lipid droplets, and if it is not effectively fixed in time, fat cell membrane damage is prone to occur during paraffin filmmaking, resulting in incomplete adipocyte structure. In the process of ice cutting and fat dyeing, fat droplet displacement is prone to occur. This product fat special fixative solution is specially used for the fixation of adipose tissue, which can effectively and timely fix fat, solve the above production problems, and prepare good quality fatty paraffin sections and frozen sections. The main components of this product are formaldehyde, glacial acetic acid and a small amount of ethanol.
Storage and Handling Conditions
Store and transport at room temperature, valid for 24 months.
Assay Protocol
The fresh adipose tissue was removed and fixed in special adipose fixation solution for more than 24 h. The volume of fixed fluid was determined to be completely immersed in the tissue and the tissue could move freely. Due to the small density of adipose tissue, appropriate amount of absorbent cotton balls can be inserted on the surface of the fixation container to make the adipose tissue sink into the fixation fluid and get full fixation.
For Research Use Only!
