Recombinant Human CD34 Protein (His-tag)

Gene Name Synonym

CD34, RP11-328D5.2

Species

Human

Accession

P28906

Source

HEK293 cells

SPECIFICATIONS

Amino acid sequence

Ser32-Thr290 is expressed with a 10×His tag at the N terminus

Molecular Weight

27.5 kDa（The protein migrates as 65-100 kDa under reducing condition (SDS-PAGE) due to glycosylation）

Purity

>95.0% as determined by SDS-PAGE

Endotoxin

<5 EU mg^-1

Formulation

Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

CD34 is a transmembrane phosphoglycoprotein, first identified on hematopoietic stem and progenitor cells. This molecule is also expressed by mature inflammatory cells such as eosinophils, mast cells, and dendritic cells, as well as on a few cells involved in ASM remodeling, such as fibrocytes and ASM precursors It has been widely used as a stem and progenitor cell marker, and clinical CD34+ stem cell transplantation (CD34+SCT) has been performed for tumor purging. CD34 is a cell-surface sialomucin.

Data

SDS-PAGE

Bioactivity

Active Recombinant Human CD34 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 65-110 kDa.

Immobilized Human CD34, His Tag at 5 μg/mL (100 μL/well) can bind Anti-CD34 with a linear range of 0.03-0.3 μg/mL.