Recombinant Rat IL-4 Protein (His-tag)

Gene Name Synonym

IL-4, IL4, BCGF1, BSF1

Species

Rat

Accession

P20096

Source

HEK293 cells

SPECIFICATIONS

Amino acid sequence

Cys25-Ser147 is expressed with a 10×His tag at the C terminus

Molecular Weight

15.2 kDa

Purity

>95.0% as determined by SDS-PAGE

Endotoxin

<5 EU mg^-1

Formulation

Lyophilized from sterile PBS, pH 7.4. Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization.

Reconstitution

Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation.

STORAGE

Stability and Storage

12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

BACKGROUND

Interleukin-4, also known as IL4, is a secreted protein that belongs to the IL-4 / IL-13 family. IL-4 was first identified as a T cell-derived growth factor for B cells. In addition to T cells, IL-4 is produced by innate lymphocytes, such as NTK cells, and myeloid cells, such as basophils and mast cells. Interleukin-4 / IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation. Interleukin-4 / IL4 also regulates the expression of the low-affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Interleukin-4 is essential for the switching of B cells to IgE antibody production and the maturation of T helper (Th) cells toward the Th2 phenotype.It participates in at least several B-cell activation processes as well as other cell types.

Data

SDS-PAGE

Bioactivity

Recombinant Rat IL-4 Protein was determined by SDS-PAGE with Coomassie Blue.

Measured in a cell proliferation assay using rat splenocytes. The ED50 for this effect is typically 5-10 ng/mL.