MEM,GlutaPlus,no HEPES, 500 mL

Attribute:

SKU : G4557-500ML

Brand : Servicebio

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Product Information

Product Name

Cat. No.

Spec.

MEM, GlutaPlus, no HEPES

G4557-500ML

500 mL

 


Product Description

MEM (Minimum Essential Medium), also known as minimum basic medium, low limit Eagle medium, is one of the most commonly used basic medium in animal cell culture. MEM contains only 12 amino acids, 8 vitamins and basic inorganic salts, with a simple nutrient composition, is mainly used for the culture of walled cells, but can also be used for other types of cell culture after the formulation is revised.

This product contains amino acids, vitamins, inorganic salts and other ingredients required for cell culture, without protein or growth factors. Need to add serum or serum-free additives depending on the cell type.

This product is filtered and sterilized by 0.1 μm filter membrane, pH 7.0-7.4, contains 5.5 mM D-glucose,  2.0 mM L-Alanyl-L-Glutamine and phenol red indicator, without sodium pyruvate, NEAA and HEPES.

 


Storage and Shipping Conditions

Ship at room temperature; Store away from light at 2-8, valid for 12 months.

 


Note

1. This product has been filtered and sterilized. Attention should be paid to aseptic operation to avoid contamination.

2. For best results, do not freeze and thaw repeatedly.

3. For your safety and health, please wear safety glasses, gloves or protective clothing.

 

For Research Use Only!

G4557 MEM Formula

Components

Concentration


Components

Concentration

mg/L

mM

mg/L

mM

Amino acids




Inorganic salts



L-Arginine hydrochloride

126.0

0.5972


Calcium Chloride (CaCl2)(anhyd.)

200

1.802

L-Cystine 2HCl

31.0

0.09904


Magnesium Sulfate heptahydrate (MgSO4·7H2O )

200

0.813

L-Alanyl-L-Glutamine

434.4

2.0


Potassium Chloride (KCl)

400

5.333

L-Histidine hydrochloride-H2O

42.0

0.20


Sodium Bicarbonate (NaHCO3)

2200

26.19

L-Isoleucine

52.0

0.3969


Sodium Chloride (NaCl)

6350

109.5

L-Leucine

52.0

0.3969


Sodium Phosphate monobasic (NaH2PO4) (anhyd.)

122

1.014

L-Lysine hydrochloride

73.0

0.3989


Other components



L-Methionine

15.0

0.1007


D-Glucose (Dextrose)

1000

5.556

L-Phenylalanine

32.0

0.1939


Phenol Red

9.4

0.02657

L-Threonine

48.0

0.4034


HEPES

-

-

L-Tryptophan

10.0

0.04902





L-Tyrosine Disodium Salt Dihydrate

52.0

0.1992





L-Valine

46.0

0.3932





Glycine

-

-





L-Alanine

-

-





L-Asparagine monohydrate

-

-





L-Aspartic Acid

-

-





L-Glutamic Acid

-

-





L-Proline

-

-





L-Serine

-

-





Vitamins







Choline chloride

1.0

0.007143





D-Calcium pantothenate

1.0

0.002096





Folic Acid

1.0

0.002268





Niacinamide

1.0

0.008197





Pyridoxine hydrochloride

1.0

0.004902





Riboflavin

0.10

0.0002660





Thiamine hydrochloride

1.0

0.002967





Meso-Inositol

2.0

0.01111





 

 

 

 

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