RPMI-1640 medium, no L-Glutamine, no HEPES, no sodium pyruvate, 500 mL
Attribute:
SKU : G4534-500ML
Categories : 2. Cell & Molecular Biology , Cell Culture Medium / Sera , Servicebio ,
Brand : Servicebio
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Product Content
Product Name | Cat. No. | Spec. |
RPMI-1640 medium no L-Glutamine,no HEPES,no sodium pyruvate | G4534-500ML | 500 mL |
Product Description
RPMI refers to Roswell Park Memorial Institute. RPMI-1640 medium was originally developed to culture human leukemic cells in suspnesion and as a monolayer. RPMI-1640 medium is unique from other media because it contains reducing glutathione and high concentrations of vitamins. Rpmi-1640 medium contains biotin, vitamin B12 and para-aminobenzoic acid, which are not found in Eagles Minimum Essential Medium or Dulbeccos Modified Eagle Medium. In addition, inositol and choline are present in very high concentrationd. Servicebio RPMI-1640 media preparations are available in with glucose or no glucose formulations, as well as with or without L-glutamine and sodium pyruvate. Servicebio RPMI-1640 media include phenol red as a pH indicator. All available RPMI-1640 media products contain a sodium bicarbonate buffer with or without HEPES to maintain optimal culture pH.
RPMI-1640 medium has been found sutiable for a variety of mammalian cells, including Hela, Juukat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas. RPMI-1640 contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI-1640 uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 510% CO2 environment to maintain physiological pH.
All raw material components used for Servicebio cell culture media products are screened through using strict quality control testing. Servicebio cell culture media are manufactured at a cGMP-compliant facility using water for injection, filtered through final 0.1 μm sterile filtration and aseptic filled in a Hundred-stage FFU. The filter integrity tests are performed before and after filtration. Each lot of cell culture media are subjected to sterility test, pH, osmolality, endotoxin and cell culture test.
The major character of this RPMI-1640 medium is as follows:
l With 11.1 mM Glucose, phenol red.
l Without L-glutamine, sodium pyruvate and HEPES.
The complete formulation is available.
Storage
Storage conditions: 2 to 8, protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
For Research Use Only!
G4534 RPMI-1640 Medium
Components | Concentration | Components | Concentration | |||
mg/L | mM | mg/L | mM | |||
Amino acids | Inorganic salts | |||||
Glycine | 10 | 0.133 | Calcium nitrate (Ca(NO3)2 4H2O) | 100 | 0.4237 | |
L-Arginine hydrochloride | 241.86 | 1.149 | Magnesium Sulfate (MgSO4) (anhyd.) | 48.84 | 0.407 | |
L-Asparagine hydrate | 56.82 | 0.379 | Potassium Chloride (KCl) | 400 | 5.33 | |
L-Aspartic Acid | 20 | 0.15 | Sodium Bicarbonate (NaHCO3) | 2000 | 23.8 | |
L-Cystine dihydrochloride | 65.2 | 0.208 | Sodium Chloride (NaCl) | 5300 | 90.6 | |
L-Glutamic Acid | 20 | 0.136 | Sodium Phosphate monobasic (NaH2PO4) (anhyd.) | 800 | 5.63 | |
L-Histidine HCl H2O | 20.22 | 0.097 | Other components | |||
L-Hydroxyproline | 20 | 0.153 | D-Glucose (Dextrose) | 2000 | 11.11 | |
L-Isoleucine | 50 | 0.382 | Sodium pyruvate | |||
L-Leucine | 50 | 0.382 | Glutathione (reduced) | 1.0 | 0.003 | |
L-Lysine hydrochloride | 40 | 0.219 | HEPES | |||
L-Methionine | 15 | 0.101 | Phenol Red | 4.6 | 0.013 | |
L-Phenylalanine | 15 | 0.091 | ||||
L-Proline | 20 | 0.174 | ||||
L-Serine | 30 | 0.286 | ||||
L-Threonine | 20 | 0.168 | ||||
L-Tryptophan | 5.0 | 0.025 | ||||
L-Tyrosine Disodium Salt Dihydrate | 28.83 | 0.111 | ||||
L-Valine | 20 | 0.171 | ||||
L-Glutamine | ||||||
L-Alanyl-L-Glutamine | ||||||
Vitamins | ||||||
Biotin | 0.2 | 0.00082 | ||||
Choline chloride | 3.0 | 0.02143 | ||||
D-Calcium pantothenate | 0.25 | 0.00052 | ||||
Folic Acid | 1.0 | 0.00227 | ||||
Niacinamide | 1.0 | 0.00820 | ||||
Para-Aminobenzoic Acid | 1.0 | 0.00730 | ||||
Pyridoxine hydrochloride | 1.0 | 0.00485 | ||||
Riboflavin | 0.2 | 0.00053 | ||||
Thiamine hydrochloride | 1.0 | 0.00297 | ||||
Vitamin B12 | 0.005 | 0.00000369 | ||||
i-Inositol | 35 | 0.19444 | ||||
